Electronic Resource
Micro-Raman Spectroscopy for Detection of Label-Free and Oil Red O Labeled PEGylated Nanoliposomes in hCmec/D3 Cell Internalization Studies
| العنوان: | Micro-Raman Spectroscopy for Detection of Label-Free and Oil Red O Labeled PEGylated Nanoliposomes in hCmec/D3 Cell Internalization Studies |
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| المؤلفون: | Gorachinov, Filip, Makreski, Petre, Shalabalija, Dushko, Mihailova, Ljubica, Simonoska Crcarevska, Maja, Glavas Dodov, Marija, Goracinova, Katerina, Jovanovski, Gligor, Geskovski, Nikola |
| المصدر: | Croatica Chemica Acta; ISSN 0011-1643 (Print); ISSN 1334-417X (Online); Volume 95; Issue 4 |
| بيانات النشر: | Croatian Chemical Society 2022 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Rapid development of nanomedicines necessitates advancement in internalization techniques which can accurately distinguish between the complex environments of cells and nanocarriers. Internalization (or endocytosis) studies of oil red O labeled and label-free PEGylated-lecithin/cholesterol nanoliposomes was performed using micro-Raman spectroscopy. The C.O stretching vibrations and CCH scissoring bendings of naphthalene ring around 1225 cm.1 as well as the N=N stretching vibrations at 1377 cm.1 are prominent peaks absent from the label-free spectra which can be used for detection of internalized oil red O labeled nanoliposomes. Suitability of oil red O as a liposome marker was confirmed by stability studies of the incorporated dye and automated fluorescence cell counting. The C.C stretching region with a prominent wide band centered at 1080 cm.1 indicative of larger gauche conformer content typical for the lecithin-cholesterol nanoliposomes and the strong maximum at 980 cm.1 associated with O.C.C.N+ stretching vibrations of the liposome polar head groups are important for studying label-free nanoliposome cell internalization. |
| مصطلحات الفهرس: | PEGylated nanoliposomes; oil red O labeled PEGylated-nanoliposomes; cell internalization studies; micro-Raman spectroscopy, text, info:eu-repo/semantics/article, info:eu-repo/semantics/publishedVersion |
| URL: | info:eu-repo/semantics/altIdentifier/doi/10.5562/cca3912 |
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| ملاحظة: | application/pdf English |
| Other Numbers: | HRCAK oai:hrcak.srce.hr:305557 1394881796 |
| المصدر المساهم: | HRCAK PORTAL ZNANSTVENIH CASOPISA REPUB From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1394881796 |
| قاعدة البيانات: | OAIster |
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