Electronic Resource
Exploitation of lichens as a source of novel natural products
| العنوان: | Exploitation of lichens as a source of novel natural products |
|---|---|
| المؤلفون: | Witts, Jessica Katherine |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Lichens can be described as an obligate mutualistic symbiosis, usually between an ascomycete fungus (the mycobiont) and either a green alga and/or cyanobacterium (the photobiont), in which a unique thallus structure is formed. Fungal species which follow this evolutionarily successful lifestyle are known to produce a diverse range of secondary metabolites, many of which are unique to lichens and are not found to be produced in other non-lichen fungal species. Lichen secondary metabolites, the majority of which are thought to be produced by the fungal partner, possess a variety of biological activities and, therefore, have the potential for biotechnological and industrial applications. However, the extremely slow growth rate associated with lichens hampers their biotechnological exploitation as it is neither feasible nor sustainable to produce lichen secondary metabolites on an industrial scale. Thus, alternative methods are required for the production of lichen secondary metabolites. This study used two approaches in an attempt to overcome this problem. The first approach involved the use of UV mutagenesis to create mutants of various lichen mycobionts (Xanthoria parietina, Lecanora chlarotera and Amandinea punctata) possessing a faster growth rate. It was planned to analyse these mutants at the genomic level in order to obtain insights into the mutations supporting higher growth rates. However, although results obtained in this study showed that exposure of X. parietina ascospores to UV-C appeared to produce potentially faster-growing mutants with at least an incremental increase in growth rate, only pilot work was conducted in this study and further elucidation of the genetic basis of this increased growth rate is required. The second approach used in this study to achieve the production and characterisation of lichen-derived secondary metabolites was that of the heterologous expression of lichen secondary metabolism genes in the non-lichen fungal expression platf |
| مصطلحات الفهرس: | Lichens, biosynthesis, metabolism genes, NRPS-like enzymes, metabolites, psychrotolerant expression platform, lichen-derived proteins., Thesis (University of Nottingham only), NonPeerReviewed |
| URL: | |
| الاتاحة: | Open access content. Open access content cc_by |
| ملاحظة: | application/pdf Witts, Jessica Katherine (2022) Exploitation of lichens as a source of novel natural products. PhD thesis, University of Nottingham. English |
| Other Numbers: | UVN oai:eprints.nottingham.ac.uk:71202 https://eprints.nottingham.ac.uk/71202/1/Thesis_JWitts_Exploitation%20of%20Lichens%20as%20a%20Source%20of%20Novel%20Natural%20Products.pdf Witts, Jessica Katherine (2022) Exploitation of lichens as a source of novel natural products. PhD thesis, University of Nottingham. 1381530534 |
| المصدر المساهم: | UNIV OF NOTTINGHAM From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1381530534 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |