Electronic Resource
Influence of chlorine dioxide on cell death and cell cycle of human gingival fibroblasts
| العنوان: | Influence of chlorine dioxide on cell death and cell cycle of human gingival fibroblasts |
|---|---|
| المؤلفون: | Nishikiori, Ryo, Nomura, Yuji, Sawajiri, Masahiko, Masuki, Kohei, Hirata, Isao, Okazaki, Masayuki |
| بيانات النشر: | Elsevier Science Ltd. 2008-12 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Objectives: The effects of chlorine dioxide (ClO2), sodium hypochlorite (NaOCl), and hydrogen peroxide (H2O2) on cell death and the cell cycle of human gingival fibroblast (HGF) cells were examined. Methods: The inhibition of HGF cell growth was evaluated using a Cell Counting Kit-8. The cell cycle was assessed with propidium iodide-stained cells (distribution of cells in G0/G1, S, G2/M phases) using flow cytometry. The patterns of cell death (necrosis and apoptosis) were analyzed using flow cytometry with annexin V-FITC/PI staining. Results: The lethal doses for 50% of the cells (LD50) of ClO2, NaOCl, and H2O2 were 0.16 mM, 0.79 mM, and 0.11 mM, respectively. All three dental disinfectants induced G0/G1 cell cycle arrest. H2O2 induced apoptosis at concentrations of 0.05 and 0.1 mM, while NaOCl and ClO2 did not induce significant apoptosis at any concentration examined. Conclusions: These results suggest that ClO2 is sufficient for use as a dental disinfectant compared with H2O2 or NaOCl. |
| مصطلحات الفهرس: | apoptotic influence, dental disinfectants, chlorine dioxide, human gingival fibroblast, Journal Article, AO |
| URL: | 0300-5712 AA00696556 |
| الاتاحة: | Open access content. Open access content open access Copyright (c) 2008 Elsevier Ltd |
| ملاحظة: | application/pdf English |
| Other Numbers: | JPNII oai:irdb.nii.ac.jp:01372:0005823490 Journal of Dentistry, 36(12), 993-998 1381439624 |
| المصدر المساهم: | NATIONAL INST OF INFO From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1381439624 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |