Electronic Resource
Microbioreactors for nutrient-controlled microbial cultures: Bridging the gap between bioprocess development and industrial use
| العنوان: | Microbioreactors for nutrient-controlled microbial cultures: Bridging the gap between bioprocess development and industrial use |
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| المؤلفون: | Totlani, K. (author), van Tatenhove-Pel, R.J. (author), Kreutzer, M.T. (author), van Gulik, W.M. (author), van Steijn, V. (author) |
| بيانات النشر: | 2023 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | It is common practice in the development of bioprocesses to genetically modify a microorganism and study a large number of resulting mutants in order to select the ones that perform best for use at the industrial scale. At industrial scale, strict nutrient-controlled growth conditions are imposed to control the metabolic activity and growth rate of the microorganism, thereby enhancing the expression of the product of interest. Although it is known that microorganisms that perform best under these strictly controlled conditions are not the same as the ones that perform best under uncontrolled batch conditions, screening, and selection is predominantly performed under batch conditions. Tools that afford high throughput on the one hand and dynamic control over cultivation conditions on the other hand are not yet available. Microbioreactors offer the potential to address this problem, resolving the gap between bioprocess development and industrial scale use. In this review, we highlight the current state-of-the-art of microbioreactors that offer the potential to screen microorganisms under dynamically controlled conditions. We classify them into: (i) microtiter plate-based platforms, (ii) microfluidic chamber-based platforms, and (iii) microfluidic droplet-based platforms. We conclude this review by discussing the opportunities of nutrient-fed microbioreactors in the field of biotechnology. ChemE/Product and Process Engineering BT/Industriele Microbiologie Architectural Engineering +Technology |
| مصطلحات الفهرس: | chemostat, fed-batch, industrially-relevant screening, microbioreactors, nutrient-limited growth, review |
| DOI: | 10.1002.biot.202200549 |
| URL: | Biotechnology Journal--1860-6768--80eb5eb9-6377-4051-9b13-cda45de92f5c |
| الاتاحة: | Open access content. Open access content © 2023 K. Totlani, R.J. van Tatenhove-Pel, M.T. Kreutzer, W.M. van Gulik, V. van Steijn |
| ملاحظة: | English |
| Other Numbers: | NLTUD oai:tudelft.nl:uuid:72fc025c-0248-45b5-88e3-3e6980d2c97a doi:10.1002/biot.202200549 1376664386 |
| المصدر المساهم: | DELFT UNIV OF TECHNOL From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1376664386 |
| قاعدة البيانات: | OAIster |
| DOI: | 10.1002.biot.202200549 |
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