Electronic Resource
Thermal treatment of magnesium particles in polylactic acid polymer films elicits the expression of osteogenic differentiation markers and lipidome profile remodeling in human adipose stem cells
| العنوان: | Thermal treatment of magnesium particles in polylactic acid polymer films elicits the expression of osteogenic differentiation markers and lipidome profile remodeling in human adipose stem cells |
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| المؤلفون: | Comunidad de Madrid, Ministerio de Ciencia e Innovación (España), Chiara, A., Dominici, F., Moren, F., Rallini, M., Tortorella, I., Ferrández-Montero, Ana, Pellegrino, Roberto M., Ferrari, Begoña, Emiliani, C., Lieblich, Marcela, Torre, L., Martino, S., Armentano, I. |
| بيانات النشر: | Elsevier 2022 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The efficacy of polylactic acid (PLA)/Magnesium (Mg)-based materials for driving stem cells toward bone tissue engineering applications requires specific Mg surface properties to modulate the interface of stem cells with the film. Here, we have developed novel PLA/Mg-based composites and explored their osteogenic differentiation potential on human adipose stem cells (hASCs). Mg-particles/polymer interface was improved by two treatments: heating in oxidative atmosphere (TT) and surface modification with a compatibilizer (PEI). Different contents of Mg particles were dispersed in PLA and composite surface and bulk properties, protein adsorption, stem cell-PLA/Mg interactions, osteogenic markers expressions, and lipids composition profile were evaluated. Mg particles were uniformly distributed on the surface and in the bulk PLA polymer. Improved and modulated particle-polymer adhesion was observed in Mg particle-treated composites. After 21 days in canonical growth culture conditions, hASCs on PLA/MgTT displayed the highest expression of the general osteogenic markers, RUNX2, SSP1, and BGLAP genes, Alkaline Phosphatase, type I Collagen, Osteopontin, and Calcium deposits. Moreover, by LC/MS QTOF mass-spectrophotometry lipidomic analysis, we found in PLA/MgTT-cells, for the first time, a remodeling of the lipid classes composition associated with the osteogenic differentiation. We ascribed these results to MgTT characteristics, which improve Mg availability and composite osteoinductive performance. |
| مصطلحات الفهرس: | PLA, Osteoconductivity, Stem cells, artículo |
| URL: | Preprint Sí info:eu-repo/grantAgreement/COMUNIDAD DE MADRID//S2018/NMT-4411 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104351GB-C21/ES/DEVELOPMENT, THROUGH A NOVEL PROCESSING, OF INNOVATIVE BIODEGRADABLE AND BIOCOMPATIBLE COMPOSITE MATERIALS FORMED BY POLYMER%2FMETAL AND METAL%2FMETAL |
| الاتاحة: | Open access content. Open access content closedAccess |
| Other Numbers: | CTK oai:digital.csic.es:10261/287222 International Journal of Biological Macromolecules 223 (Part A): 684-701 (2022) 10.1016/j.ijbiomac.2022.11.005 1373149806 |
| المصدر المساهم: | CSIC From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1373149806 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |