Electronic Resource
Genome-Wide Association Study Demonstrates the Role Played by the CD226 Gene in Rasa Aragonesa Sheep Reproductive Seasonality
| العنوان: | Genome-Wide Association Study Demonstrates the Role Played by the CD226 Gene in Rasa Aragonesa Sheep Reproductive Seasonality |
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| المؤلفون: | Ministerio de Ciencia y Tecnología (España), Gobierno de Aragón, European Commission, Lakhssassi, Kenza [0000-0002-9793-7494], Lahoz, Belén [0000-0001-9394-5027], Sarto, María Pilar [0000-0003-1486-3950], Iguácel, Laura Pilar [0000-0001-6042-3134], Folch, Josep María [0000-0003-3689-1303], Alabart, J. L. [0000-0001-5015-8103], Serrano, Magdalena [0000-0002-1621-3102], Calvo, Jorge Hugo [0000-0001-9513-0219], Lakhssassi, Kenza, Lahoz, Belén, Sarto, María Pilar, Iguácel, Laura Pilar, Folch, Josep María, Alabart, José Luis, Serrano, Magdalena, Calvo, Jorge Hugo |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute 2021-04-19 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | A genome-wide association study (GWAS) was used to identify genomic regions influ encing seasonality reproduction traits in Rasa Aragonesa sheep. Three traits associated with either ovarian function based on blood progesterone levels (total days of anoestrus and progesterone cycling months) or behavioral signs of oestrous (oestrous cycling months) were studied. The GWAS included 205 ewes genotyped using the 50k and 680k Illumina Ovine Beadchips. Only one SNP associated with the progesterone cycling months overcame the genome-wide significance level (rs404991855). Nine SNPs exhibited significant associations at the chromosome level, being the SNPs rs404991855 and rs418191944, that are located in the CD226 molecule (CD226) gene, associated with the three traits. This gene is related to reproductive diseases. Two other SNPs were located close to the neuropeptide Y (NPY) gene, which is involved in circadian rhythms. To validate the GWAS, partial characterization of both genes by Sanger sequencing, and genotyping of two synonymous and two nonsynonymous SNPs in the NPY and CD226 genes, respectively, were performed. SNP association analysis showed that only SNP rs404360094 in the exon 3 of the CD226 gene, which produces an amino acid substitution from asparagine (uncharged polar) to aspartic acid (acidic), was associated with the three seasonality traits. Our results suggest that the CD226 gene may be involved in the reproductive seasonality in Rasa Aragonesa. |
| مصطلحات الفهرس: | CD226, GWAS, NPY, Oestrous, Reproduction; reproduction; seasonality, Reproduction, Seasonality, artículo |
| URL: | Animals : an open access journal from MDPI Publisher's version Sí info:eu-repo/grantAgreement/MICIU//RTA2015-00090 info:eu-repo/grantAgreement/EC/A14-20R info:eu-repo/grantAgreement/MICIU//BES-2017-080154 |
| الاتاحة: | Open access content. Open access content https:// creativecommons.org/licenses/by/ 4.0 openAccess |
| ملاحظة: | English |
| Other Numbers: | CTK oai:digital.csic.es:10261/285207 Animals 11(4): 1171 (2021) 2076-2615 10.3390/ani11041171 33921837 2-s2.0-85104419021 1356201594 |
| المصدر المساهم: | CSIC From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1356201594 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |