Electronic Resource
Cariogenicity activity and related gene expression of cariogenic bacteria in dental biofilm of asthmatic patients
| العنوان: | Cariogenicity activity and related gene expression of cariogenic bacteria in dental biofilm of asthmatic patients |
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| المؤلفون: | Sitthichai Khunthongkaew, advisor, Kusumawadee Utispan, co-advisor, Widhianingsih, Dhyani |
| بيانات النشر: | Thammasat University 2019 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Background: Both of dental caries and asthma are multifactorial diseases, this highlights the importance for dental practitioners to evaluate the risk of caries in asthma patients so that prevention can be anticipated. To our knowledge, this study is the first report on evaluating cariogenic dental plaque using three-tone disclosing agent and expression of caries virulence gene in dental plaque samples of asthma patients. Material and methods: In the cross-sectional study, subjects were divided into 2 groups, including group of 38 asthma patients and group of 22 healthy subjects with an age range between 6-60 years. Data collection was included demographic data, asthma characteristic, use of medication, oral health behavior, and dietary habits using questionnaire and interview. The dental plaque maturity in the asthma and healthy group were assessed using GC Tri Plaque ID gel. The stained plaque was collected and used to determine the expression of caries virulence genes including spaP, gtfB, gbpB, ldh, brpA, and luxS genes by real-time PCR. The expression level of gene was normalized with 16S rRNA as an internal control gene.Result: Asthmatic had a higher percentage of mature and acidogenic plaque compared to immature plaque. In contrast immature plaque was obviously found in controls. Acidogenic plaque was obviously found in the patients who used 1 or combination of 2 medications. High frequency in food and sugar intakes were found in asthmatic. Real-time PCR revealed that the expression of spaP, gtfB, gbpB, ldh, brpA, and luxS genes increased in asthmatic compared to healthy group. Conclusion: An increase in acidogenic and mature plaque was found in asthma patients. The expression of spaP, gtfB, gbpB, ldh, brpA, and luxS genes in detal plaque were upregulated in asthmatics |
| مصطلحات الفهرس: | Asthma, Caries, GC Tri plaque ID Gel, SpaP, GtfB, GbpB, Ldh, BrpA, LuxS, Text |
| URL: | |
| الاتاحة: | Open access content. Open access content Copyright of Thammasat University. Licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives license. |
| ملاحظة: | application/pdf 15, 99 leaves English |
| Other Numbers: | TULIB 187310 1346251155 |
| المصدر المساهم: | THAMMASAT UNIV LIBR From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1346251155 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |