Electronic Resource
Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging
| العنوان: | Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging |
|---|---|
| المؤلفون: | Qian, Yong, Cosio, Danielle M. Orozco, Piatkevich, Kiryl D., Aufmkolk, Sarah, Su, Wan-Chi, Celiker, Orhan T., Schohl, Anne, Murdock, Mitchell H., Aggarwal, Abhi, Chang, Yu-Fen, Wiseman, Paul W., Ruthazer, Edward S., Boyden, Edward S., Campbell, Robert E. |
| المصدر: | PLoS |
| بيانات النشر: | Public Library of Science (PLoS) 2022-06-13T15:05:55Z 2021-10-27T19:57:46Z 2022-06-13T15:05:55Z 2020-11 2020-04 2021-03-12T15:47:32Z |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | © 2020 Qian et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Near-infrared (NIR) genetically encoded calcium ion (Ca2+) indicators (GECIs) can provide advantages over visible wavelength fluorescent GECIs in terms of reduced phototoxicity, minimal spectral cross talk with visible light excitable optogenetic tools and fluorescent probes, and decreased scattering and absorption in mammalian tissues. Our previously reported NIR GECI, NIR-GECO1, has these advantages but also has several disadvantages including lower brightness and limited fluorescence response compared to state-of-the-art visible wavelength GECIs, when used for imaging of neuronal activity. Here, we report 2 improved NIR GECI variants, designated NIR-GECO2 and NIR-GECO2G, derived from NIR-GECO1. We characterized the performance of the new NIR GECIs in cultured cells, acute mouse brain slices, and Caenorhabditis elegans and Xenopus laevis in vivo. Our results demonstrate that NIR-GECO2 and NIR-GECO2G provide substantial improvements over NIR-GECO1 for imaging of neuronal Ca2+ dynamics. |
| مصطلحات الفهرس: | Article, http://purl.org/eprint/type/JournalArticle |
| URL: | PLoS Biology |
| الاتاحة: | Open access content. Open access content Creative Commons Attribution 4.0 International license https://creativecommons.org/licenses/by/4.0 |
| ملاحظة: | application/octet-stream English |
| Other Numbers: | MYG oai:dspace.mit.edu:1721.1/134043.2 1545-7885 1342472429 |
| المصدر المساهم: | MASSACHUSETTS INST OF TECHNOL LIBRS From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1342472429 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |