Electronic Resource
Cellular extrusion bioprinting improves kidney organoid reproducibility and conformation
| العنوان: | Cellular extrusion bioprinting improves kidney organoid reproducibility and conformation |
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| المؤلفون: | Lawlor, KT, Vanslambrouck, JM, Higgins, JW, Chambon, A, Bishard, K, Arndt, D, Er, PX, Wilson, SB, Howden, SE, Tan, KS, Li, F, Hale, LJ, Shepherd, B, Pentoney, S, Presnell, SC, Chen, AE, Little, MH |
| بيانات النشر: | NATURE PORTFOLIO 2021-02 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Directed differentiation of human pluripotent stem cells to kidney organoids brings the prospect of drug screening, disease modelling and the generation of tissue for renal replacement. Currently, these applications are hampered by organoid variability, nephron immaturity, low throughput and limited scale. Here, we apply extrusion-based three-dimensional cellular bioprinting to deliver rapid and high-throughput generation of kidney organoids with highly reproducible cell number and viability. We demonstrate that manual organoid generation can be replaced by 6- or 96-well organoid bioprinting and evaluate the relative toxicity of aminoglycosides as a proof of concept for drug testing. In addition, three-dimensional bioprinting enables precise manipulation of biophysical properties, including organoid size, cell number and conformation, with modification of organoid conformation substantially increasing nephron yield per starting cell number. This facilitates the manufacture of uniformly patterned kidney tissue sheets with functional proximal tubular segments. Hence, automated extrusion-based bioprinting for kidney organoid production delivers improvements in throughput, quality control, scale and structure, facilitating in vitro and in vivo applications of stem cell-derived human kidney tissue. |
| مصطلحات الفهرس: | Journal Article |
| URL: | |
| الاتاحة: | Open access content. Open access content |
| Other Numbers: | UMV oai:jupiter.its.unimelb.edu.au:11343/278594 Lawlor, K. T., Vanslambrouck, J. M., Higgins, J. W., Chambon, A., Bishard, K., Arndt, D., Er, P. X., Wilson, S. B., Howden, S. E., Tan, K. S., Li, F., Hale, L. J., Shepherd, B., Pentoney, S., Presnell, S. C., Chen, A. E. & Little, M. H. (2021). Cellular extrusion bioprinting improves kidney organoid reproducibility and conformation. NATURE MATERIALS, 20 (2), pp.260-+. https://doi.org/10.1038/s41563-020-00853-9. 10.1038/s41563-020-00853-9 1476-4660 1476-1122 1315690920 |
| المصدر المساهم: | UNIV OF MELBOURNE From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1315690920 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |