Electronic Resource
Toxicity of differently sized and charged silver nanoparticles to yeast Saccharomyces cerevisiae BY4741: a nano-biointeraction perspective
| العنوان: | Toxicity of differently sized and charged silver nanoparticles to yeast Saccharomyces cerevisiae BY4741: a nano-biointeraction perspective |
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| المؤلفون: | Kasemets, K, Kaosaar, S, Vija, H, Fascio, U, Mantecca, P, Kasemets K., Kaosaar S., Vija H., Fascio U., Mantecca P. |
| بيانات النشر: | Taylor and Francis Ltd 2019 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | In the current study, we evaluated the modulatory effects of size and surface coating/charge of AgNPs on their toxicity to a unicellular yeast Saccharomyces cerevisiae BY4741–a fungal model. For that, the toxicity of a set of 10 and 80 nm citrate-coated (negatively charged) and branched polyethylenimine (bPEI) coated (positively charged) AgNPs was evaluated in parallel with AgNO3 as ionic control. Yeast cells were exposed to different concentrations of studied compounds in deionized water for 24 h at 30 °C and evaluated for the viability by the post-exposure colony-forming ability. Particle-cell interactions were assessed by SEM, TEM and confocal laser scanning microscopy (CLSM) in the reflection mode. AgNPs toxicity to yeast was size and charge-dependent: 24-h IC50 values ranged from 0.04 (10nAg-bPEI) up to 8.3 mg Ag/L (80nAg-Cit). 10 nm AgNPs were 5–27 times more toxic than 80 nm AgNPs and bPEI-AgNPs 8–44 times more toxic than citrate-AgNPs. SEM and TEM visualization showed that bPEI-AgNPs but not citrate-AgNPs adsorbed onto the yeast cell’s surface. However, according to CLSM all the studied AgNPs, whatever the size and coating, ended up within the yeast cell. Toxicity of citrate-AgNPs was largely explained by the dissolved Ag ions but the bPEI-AgNPs showed mainly particle-driven effects leading to the cellular internalization and/or to more pronounced dissolution of AgNPs in the close vicinity of the cell wall. Therefore, the size, and especially the coating/charge of AgNPs can be efficiently used for the design of new more efficient antifungals. |
| مصطلحات الفهرس: | cell structure, confocal and electron microscopy, Silver nanoparticle, toxicity, info:eu-repo/semantics/article |
| URL: | info:eu-repo/semantics/altIdentifier/pmid/31107118 info:eu-repo/semantics/altIdentifier/wos/WOS:000472327000001 volume:13 issue:8 firstpage:1041 lastpage:1059 numberofpages:19 journal:NANOTOXICOLOGY |
| الاتاحة: | Open access content. Open access content |
| ملاحظة: | English |
| Other Numbers: | ITBAO oai:boa.unimib.it:10281/262749 10.1080/17435390.2019.1621401 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85067846781 1308932044 |
| المصدر المساهم: | BICOCCA OPEN ARCH From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1308932044 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |