Electronic Resource
Diagnostic application of kidney allograft-derived absolute cell-free DNA levels during transplant dysfunction.
| العنوان: | Diagnostic application of kidney allograft-derived absolute cell-free DNA levels during transplant dysfunction. |
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| المؤلفون: | Bruno D.L., Slater H.R., Whitlam J.B., Ling L., Skene A., Kanellis J., Ierino F.L., Power D.A. |
| بيانات النشر: | Blackwell Publishing Ltd United Kingdom 2019-04-02 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Graft-derived cell-free DNA (donor-derived cell-free DNA) is an emerging marker of kidney allograft injury. Studies examining the clinical validity of this biomarker have previously used the graft fraction, or proportion of total cell-free DNA that is graft-derived. The present study evaluated the diagnostic validity of absolute measurements of graft-derived cell-free DNA, as well as calculated graft fraction, for the diagnosis of graft dysfunction. Plasma graft-derived cell-free DNA, total cell-free DNA, and graft fraction were correlated with biopsy diagnosis as well as individual Banff scores. Sixty-one samples were included in the analysis. For the diagnosis of antibody mediated rejection, the receiver-operator characteristic area under the curves of graft-derived cell-free DNA and graft fraction were 0.91 (95% CI 0.82-0.98) and 0.89 (95% CI 0.79-0.98), respectively. Both measures did not diagnose borderline or type 1A cellular mediated rejection. Graft fraction was associated with a broader range of Banff lesions, including lesions associated with cellular mediated rejection, while graft-derived cell-free DNA appeared more specific for antibody mediated rejection. Limitations of this study include a small sample size and lack of a validation cohort. The capacity for absolute quantification, and lower barriers to implementation of this methodology recommend it for further study.Copyright © 2018 The American Society of Transplantation and the American Society of Transplant Surgeons |
| مصطلحات الفهرس: | renal graft dysfunction/dt [Drug Therapy], cyclosporine/dt [Drug Therapy], DNA/ec [Endogenous Compound], mammalian target of rapamycin/dt [Drug Therapy], mycophenolic acid/dt [Drug Therapy], OKT 3/dt [Drug Therapy], prednisolone/dt [Drug Therapy], tacrolimus/dt [Drug Therapy], adult, antibody mediated rejection, antibody specificity, article, blood sampling, cohort analysis, comparative study, controlled study, creatinine blood level, cross-sectional study, diagnostic test accuracy study, droplet digital polymerase chain reaction, female, genotype, histopathology, human, human tissue, immunosuppressive treatment, kidney allograft, major clinical study, priority journal, prospective study, proteinuria, renal graft dysfunction/di [Diagnosis], male, renal graft dysfunction/pc [Prevention], sensitivity and specificity, azathioprine/dt [Drug Therapy], basiliximab/dt [Drug Therapy], creatinine/ec [Endogenous Compound], Article |
| URL: | American Journal of Transplantation LibKey Link |
| الاتاحة: | Open access content. Open access content Copyright 2019 Elsevier B.V., All rights reserved. |
| Other Numbers: | AUSHL oai:repository.monashhealth.org:1/35734 American Journal of Transplantation. 19 (4) (pp 1037-1049), 2019. Date of Publication: April 2019. 1600-6135 https://repository.monashhealth.org/monashhealthjspui/handle/1/35734 30312536 [http://www.ncbi.nlm.nih.gov/pubmed/?term=30312536] 624871394 (Whitlam, Power) Department of Nephrology, Austin Health, Melbourne, VIC, Australia (Whitlam, Ling, Slater) Cyto-molecular Diagnostics Research Melbourne, Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne, VIC, Australia (Whitlam, Ierino, Power) Department of Medicine, University of Melbourne, Melbourne, VIC, Australia (Skene) Department of Anatomical Pathology, Austin Health, Melbourne, VIC, Australia (Kanellis) Department of Nephrology, Department of Medicine, Monash Health and Centre for Inflammatory Diseases, Monash University, Melbourne, VIC, Australia (Ierino) Department of Nephrology, St Vincent's Health, Melbourne, VIC, Australia (Slater) Department of Paediatrics, The University of Melbourne, Melbourne, VIC, Australia (Bruno) Victorian Clinical Genetics Services Melbourne, Murdoch Children's Research Institute, Melbourne, VIC, Australia Whitlam J.B.; john.whitlam@austin.org.au 1305123256 |
| المصدر المساهم: | MONASH HEALTH LIBRS From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1305123256 |
| قاعدة البيانات: | OAIster |
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