Electronic Resource
Biophysical Characterization of Pro-apoptotic BimBH3 Peptides Reveals an Unexpected Capacity for Self-Association
| العنوان: | Biophysical Characterization of Pro-apoptotic BimBH3 Peptides Reveals an Unexpected Capacity for Self-Association |
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| المؤلفون: | German Research Foundation, National Health and Medical Research Council (Australia), Assafa, Tufa E., Nandi, Sukhendu, Śmiłowicz, Dariusz, Galazzo, Laura, Teucher, Markus, Elsner, Christina, Pütz, Stefanie, Bleicken, Stephanie, Robin, Adeline Y., Westphal, Dana, Usón, Isabel, Stoll, Raphael, Czabotar, Peter E., Metzler-Nolte, Nils, Bordignon, Enrica |
| بيانات النشر: | Elsevier 2021-02-04 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Bcl-2 proteins orchestrate the mitochondrial pathway of apoptosis, pivotal for cell death. Yet, the structural details of the conformational changes of pro- and antiapoptotic proteins and their interactions remain unclear. Pulse dipolar spectroscopy (double electron-electron resonance [DEER], also known as PELDOR) in combination with spin-labeled apoptotic Bcl-2 proteins unveils conformational changes and interactions of each protein player via detection of intra- and inter-protein distances. Here, we present the synthesis and characterization of pro-apoptotic BimBH3 peptides of different lengths carrying cysteines for labeling with nitroxide or gadolinium spin probes. We show by DEER that the length of the peptides modulates their homo-interactions in the absence of other Bcl-2 proteins and solve by X-ray crystallography the structure of a BimBH3 tetramer, revealing the molecular details of the inter-peptide interactions. Finally, we prove that using orthogonal labels and three-channel DEER we can disentangle the Bim-Bim, Bcl-xL-Bcl-xL, and Bim-Bcl-xL interactions in a simplified interactome. |
| مصطلحات الفهرس: | EPR, Apoptosis, Bim peptides, BH3, DEER, X-ray, Bcl-xL, artículo |
| URL: | Sí |
| الاتاحة: | Open access content. Open access content closedAccess |
| Other Numbers: | CTK oai:digital.csic.es:10261/254385 Structure 29(2): 114-124.e3 (2021) 0969-2126 10.1016/j.str.2020.09.002 1878-4186 1286585628 |
| المصدر المساهم: | CSIC From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1286585628 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |