Electronic Resource
Analysis of genetic diversity in Indian isolates of Rhipicephalus microplus based on Bm86 gene sequence
| العنوان: | Analysis of genetic diversity in Indian isolates of Rhipicephalus microplus based on Bm86 gene sequence |
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| المؤلفون: | Indian Council of Agricultural Research, National Agricultural Research Foundation, University Grants Commission (India), Indian Veterinary Research Institute, Chandrasekhar Parthasarathi, Balasamudram, Kumar, Binod, Nagar, Gaurav, Manjunathachar, H. V., Fuente, José de la, Ghosh, Srikant |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute 2021 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The control of cattle tick, Rhipicephalus microplus, is focused on repeated use of acaricides. However, due to growing acaricide resistance and residues problem, immunization of animals along with limited use of effective acaricides is considered a suitable option for the control of tick infestations. To date, more than fifty vaccine candidates have been identified and tested worldwide, but two vaccines were developed using the extensively studied candidate, Bm86. The main reason for limited vaccine commercialization in other countries is genetic diversity in the Bm86 gene leading to considerable variation in vaccine efficacy. India, with 193.46 million cattle population distributed in 28 states and 9 union territories, is suffering from multiple tick infestation dominated by R. microplus. As R. microplus has developed multi-acaricide resistance, an efficacious vaccine may provide a sustainable intervention for tick control. Preliminary experiments revealed that the presently available commercial vaccine based on the BM86 gene is not efficacious against Indian strain. In concert with the principle of reverse vaccinology, genetic polymorphism of the Bm86 gene within Indian isolates of R. microplus was studied. A 578 bp conserved nucleotide sequences of Bm86 from 65 R. microplus isolates collected from 9 Indian states was sequenced and revealed 95.6–99.8% and 93.2–99.5% identity in nucleotides and amino acids sequences, respectively. The identities of nucleotides and deduced amino acids were 94.7–99.8% and 91.8–99.5%, respectively, between full-length sequence (orf) of the Bm86 gene of IVRI-I strain and published sequences of vaccine strains. Six nucleotides deletion were observed in Indian Bm86 sequences. Four B-cell epitopes (D519-K554, H563-Q587, C598-T606, T609-K623), which are present in the conserved region of the IVRI-I Bm86 sequence, were selected. The results confirm that the use of available commercial Bm86 vaccines is not a suitable option against Indian is |
| مصطلحات الفهرس: | Tick, Cattle, Rhipicephalus microplus, Tick control, Bm86 gene, In silico analysis, Diversity, artículo |
| URL: | Publisher's version Sí |
| الاتاحة: | Open access content. Open access content https://creativecommons.org/licenses/by/4.0 openAccess |
| ملاحظة: | English |
| Other Numbers: | CTK oai:digital.csic.es:10261/250875 Vaccines 9(3): 194 (2021) 10.3390/vaccines9030194 2076-393X 33652549 1286582340 |
| المصدر المساهم: | CSIC From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1286582340 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |