Electronic Resource
Production of extracellular heterologous proteins in Streptomyces rimosus, producer of the antibiotic oxytetracycline
| العنوان: | Production of extracellular heterologous proteins in Streptomyces rimosus, producer of the antibiotic oxytetracycline |
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| المؤلفون: | Ministry of Higher Education Scientific and Technology (Slovenia), Universidad de Cantabria, Carrillo Rincón, Andrés Felipe, Magdevska, Vasilka, Kranjc, Luka, Fujs, Štefan, Müller, Rolf, Petković, Hrvoje |
| بيانات النشر: | Springer Nature 2018 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Among the Streptomyces species, Streptomyces lividans has often been used for the production of heterologous proteins as it can secrete target proteins directly into the culture medium. Streptomyces rimosus, on the other hand, has for long been used at an industrial scale for oxytetracycline production, and it holds ‘Generally Recognised As Safe’ status. There are a number of properties of S. rimosus that make this industrial strain an attractive candidate as a host for heterologous protein production, including (1) rapid growth rate; (2) growth as short fragments, as for Escherichia coli; (3) high efficiency of transformation by electroporation; and (4) secretion of proteins into the culture medium. In this study, we specifically focused our efforts on an exploration of the use of the Sec secretory pathway to export heterologous proteins in a S. rimosus host. We aimed to develop a genetic tool kit for S. rimosus and to evaluate the extracellular production of target heterologous proteins of this industrial host. This study demonstrates that S. rimosus can produce the industrially important enzyme phytase AppA extracellularly, and analogous to E. coli as a host, application of His-Tag/Ni-affinity chromatography provides a simple and rapid approach to purify active phytase AppA in S. rimosus. We thus demonstrate that S. rimosus can be used as a potential alternative protein expression system. |
| مصطلحات الفهرس: | Streptomyces rimosus, Heterologous protein production, Gene expression, Gene tools, Reporter system, Phytase, artículo |
| URL: | Sí |
| الاتاحة: | Open access content. Open access content closedAccess |
| Other Numbers: | CTK oai:digital.csic.es:10261/249042 Applied Microbiology and Biotechnology 102: 2607- 2620 (2018) 10.1007/s00253-018-8793-z 1286580853 |
| المصدر المساهم: | CSIC From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1286580853 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |