Electronic Resource
Gene validation and remodelling using proteogenomics of Phytophthora cinnamomi, the causal agent of dieback
| العنوان: | Gene validation and remodelling using proteogenomics of Phytophthora cinnamomi, the causal agent of dieback |
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| المؤلفون: | Andronis, C.E., Hane, J.K., Bringans, S., Hardy, G.E.S.J., Jacques, S., Lipscombe, R., Tan, K-C |
| المصدر: | Andronis, C.E., Hane, J.K. < |
| بيانات النشر: | Frontiers 2021 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Phytophthora cinnamomi is a pathogenic oomycete that causes plant dieback disease across a range of natural ecosystems and in many agriculturally important crops on a global scale. An annotated draft genome sequence is publicly available (JGI Mycocosm) and suggests 26,131 gene models. In this study, soluble mycelial, extracellular (secretome), and zoospore proteins of P. cinnamomi were exploited to refine the genome by correcting gene annotations and discovering novel genes. By implementing the diverse set of sub-proteomes into a generated proteogenomics pipeline, we were able to improve the P. cinnamomi genome annotation. Liquid chromatography mass spectrometry was used to obtain high confidence peptides with spectral matching to both the annotated genome and a generated 6-frame translation. Two thousand seven hundred sixty-four annotations from the draft genome were confirmed by spectral matching. Using a proteogenomic pipeline, mass spectra were used to edit the P. cinnamomi genome and allowed identification of 23 new gene models and 60 edited gene features using high confidence peptides obtained by mass spectrometry, suggesting a rate of incorrect annotations of 3% of the detectable proteome. The novel features were further validated by total peptide support, alongside functional analysis including the use of Gene Ontology and functional domain identification. We demonstrated the use of spectral data in combination with our proteogenomics pipeline can be used to improve the genome annotation of important plant diseases and identify missed genes. This study presents the first use of spectral data to edit and manually annotate an oomycete pathogen. |
| مصطلحات الفهرس: | Journal Article |
| URL: | full_text_status:public |
| الاتاحة: | Open access content. Open access content © 2021 The Authors. |
| ملاحظة: | English |
| Other Numbers: | LD1 oai:researchrepository.murdoch.edu.au:61892 https://researchrepository.murdoch.edu.au/id/eprint/61892/ 1276818884 |
| المصدر المساهم: | MURDOCH UNIV LIBR From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1276818884 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |