Electronic Resource
Toward the Standardization of Mitochondrial Proteomics: The Italian Mitochondrial Human Proteome Project Initiative
| العنوان: | Toward the Standardization of Mitochondrial Proteomics: The Italian Mitochondrial Human Proteome Project Initiative |
|---|---|
| المؤلفون: | Alberio, T., Pieroni, L., Ronci, M., Banfi, C., Bongarzone, I., Bottoni, P., Brioschi, M., Caterino, M., Chinello, C., Cormio, A., Cozzolino, F., Cunsolo, V., Fontana, S., Garavaglia, B., Giusti, L., Greco, Viviana, Lucacchini, A., Maffioli, E., Magni, F., Monteleone, F., Monti, M., Monti, V., Musicco, C., Petrosillo, G., Porcelli, V., Saletti, R., Scatena, Roberto, Soggiu, A., Tedeschi, G., Zilocchi, M., Roncada, P., Urbani, Andrea, Fasano, M., Greco V. (ORCID:0000-0003-4521-0020), Scatena R. (ORCID:0000-0002-9425-8293), Urbani A. (ORCID:0000-0001-9168-3174) |
| بيانات النشر: | American Chemical Society 2017 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The Mitochondrial Human Proteome Project aims at understanding the function of the mitochondrial proteome and its crosstalk with the proteome of other organelles. Being able to choose a suitable and validated enrichment protocol of functional mitochondria, based on the specific needs of the downstream proteomics analysis, would greatly help the researchers in the field. Mitochondrial fractions from ten model cell lines were prepared using three enrichment protocols and analyzed on seven different LC-MS/MS platforms. All data were processed using neXtProt as reference database. The data are available for the Human Proteome Project purposes through the ProteomeXchange Consortium with the identifier PXD007053. The processed data sets were analyzed using a suite of R routines to perform a statistical analysis and to retrieve subcellular and submitochondrial localizations. Although the overall number of identified total and mitochondrial proteins was not significantly dependent on the enrichment protocol, specific line to line differences were observed. Moreover, the protein lists were mapped to a network representing the functional mitochondrial proteome, encompassing mitochondrial proteins and their first interactors. More than 80% of the identified proteins resulted in nodes of this network but with a different ability in coisolating mitochondria-associated structures for each enrichment protocol/cell line pair. |
| مصطلحات الفهرس: | enrichment protocol, mitochondria, Mitochondrial Human Proteome Project, standardization, Cell Line, Chromatography, Liquid, Humans, Italy, Mitochondria, Mitochondrial Proteins, Protein Interaction Maps, Proteome, Proteomics, Tandem Mass Spectrometry, Settore BIO/10 - BIOCHIMICA, info:eu-repo/semantics/article |
| URL: | info:eu-repo/semantics/altIdentifier/pmid/28828861 info:eu-repo/semantics/altIdentifier/wos/WOS:000417228900008 volume:16 issue:12 firstpage:4319 lastpage:4329 numberofpages:11 issueyear:2017 journal:JOURNAL OF PROTEOME RESEARCH |
| الاتاحة: | Open access content. Open access content |
| ملاحظة: | English |
| Other Numbers: | SYC oai:publicatt.unicatt.it:10807/162608 10.1021/acs.jproteome.7b00350 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85037328540 1242037671 |
| المصدر المساهم: | UNIV CATTOLICA DEL SACRO CUORE From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1242037671 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |