Electronic Resource
Quantification of lactoyl-CoA (lactyl-CoA) by liquid chromatography mass spectrometry in mammalian cells and tissues.
| العنوان: | Quantification of lactoyl-CoA (lactyl-CoA) by liquid chromatography mass spectrometry in mammalian cells and tissues. |
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| المؤلفون: | Varner, Erika L, Trefely, Sophie, Bartee, David, von Krusenstiern, Eliana, Izzo, Luke, Bekeova, Carmen, O'Connor, Roddy S, Seifert, Erin L, Wellen, Kathryn E, Meier, Jordan L, Snyder, Nathaniel W |
| المصدر: | Department of Pathology, Anatomy, and Cell Biology Faculty Papers |
| بيانات النشر: | Jefferson Digital Commons 2020-09-01T07:00:00Z |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Lysine lactoylation is a recently described protein post-translational modification (PTM). However, the biochemical pathways responsible for this acylation remain unclear. Two metabolite-dependent mechanisms have been proposed: enzymatic histone lysine lactoylation derived from lactoyl-coenzyme A (lactoyl-CoA, also termed lactyl-CoA), and non-enzymatic lysine lactoylation resulting from acyl-transfer via lactoyl-glutathione. While the former has precedent in the form of enzyme-catalysed lysine acylation, the lactoyl-CoA metabolite has not been previously quantified in mammalian systems. Here, we use liquid chromatography-high-resolution mass spectrometry (LC-HRMS) together with a synthetic standard to detect and validate the presence of lactoyl-CoA in cell and tissue samples. Conducting a retrospective analysis of data from previously analysed samples revealed the presence of lactoyl-CoA in diverse cell and tissue contexts. In addition, we describe a biosynthetic route to generate 13C315N1-isotopically labelled lactoyl-CoA, providing a co-eluting internal standard for analysis of this metabolite. We estimate lactoyl-CoA concentrations of 1.14 × 10-8 pmol per cell in cell culture and 0.0172 pmol mg-1 tissue wet weight in mouse heart. These levels are similar to crotonyl-CoA, but between 20 and 350 times lower than predominant acyl-CoAs such as acetyl-, propionyl- and succinyl-CoA. Overall our studies provide the first quantitative measurements of lactoyl-CoA in metazoans, and provide a methodological foundation for the interrogation of this novel metabolite in biology and disease. |
| مصطلحات الفهرس: | LC-HRMS, high resolution, lactoyl-CoA, lactyl-CoA, metabolism, Anatomy, Cells, Medicine and Health Sciences, Pathology, article |
| URL: | |
| الاتاحة: | Open access content. Open access content http://creativecommons.org/licenses/by/4.0 |
| ملاحظة: | application/pdf English |
| Other Numbers: | TVJ oai:jdc.jefferson.edu:pacbfp-1311 1226331105 |
| المصدر المساهم: | THOMAS JEFFERSON UNIV From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1226331105 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |