Electronic Resource
miR-4999-5p Predicts Colorectal Cancer Survival Outcome and Reprograms Glucose Metabolism by Targeting PRKAA2
| العنوان: | miR-4999-5p Predicts Colorectal Cancer Survival Outcome and Reprograms Glucose Metabolism by Targeting PRKAA2 |
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| المؤلفون: | Zhang,Qiwei, Hong,Zhi, Zhu,Jieyao, Zeng,Chao, Tang,Zhen, Wang,Weiqiang, Huang,He |
| بيانات النشر: | Dove Press 2020-02-11 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Qiwei Zhang,1 Zhi Hong,1 Jieyao Zhu,2 Chao Zeng,1 Zhen Tang,1 Weiqiang Wang,1 He Huang1 1Department of Gastrointestinal Surgery, The First Affiliated Hospital, Yijishan Hospital of Wannan Medical College, Wuhu 241000, Anhui, People’s Republic of China; 2Department of General Surgery, Lujiang County People’s Hospital, Hefei 231500, Anhui, People’s Republic of ChinaCorrespondence: He HuangDepartment of Gastrointestinal Surgery, The First Affiliated Hospital, Yijishan Hospital of Wannan Medical College, 2 Zheshan West Road, Jinhu District, Wuhu 241000, Anhui, People’s Republic of ChinaTel +86 553 5739558Fax +86 553 5738279Email huanghwnmc@sina.comPurpose: Colorectal cancer (CRC) is the third most common cancer, and the second leading cause of cancer death worldwide. Dysregulation of microRNAs has been shown to modulate glucose metabolic reprogramming in CRC. However, the functional role of miR-4999-5p in the CRC glucose metabolic shift has not been characterized.Patients and Methods: The levels of miR-4999-5p and PRKAA2 were evaluated by RT-qPCR. Univariate and multivariate survival analyses were conducted to evaluate the prognostic value of miR-4999-5p. Cell proliferation was assessed using the CCK-8 and colony formation assays. Extracellular acidification rate, glucose uptake, cellular glucose-6-phosphate level, and lactate production were evaluated to assess the effects of miR-4999-5p on CRC glycolysis. Dual-luciferase reporter assay was conducted to investigate the direct interaction between miR-4999-5p and PRKAA2. Mouse xenograft models were established to assess the functions of miR-4999-5p in vivo.Results: miR-4999-5p was highly expressed in CRC tissues and cell lines. In addition, miR-4999-5p was associated with tumor differentiation and TNM stage, and elevated expression of miR-4999-5p was an independent predictor of poorer overall survival. Furthermore, miR-4999-5p promoted cell proliferation and glycolysis in CRC. miR |
| مصطلحات الفهرس: | OncoTargets and Therapy, Original Research, info:eu-repo/semantics/article |
| URL: | info:eu-repo/semantics/altIdentifier/doi/10.2147/OTT.S234666 |
| الاتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | text/html English |
| Other Numbers: | NZDMP oai:dovepress.com/51736 1141816905 |
| المصدر المساهم: | DOVE MEDL PRESS LTD From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1141816905 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |