Electronic Resource
MALDI-TOF MS as a tool to identify foodborne yeasts and yeast-like fungi
| العنوان: | MALDI-TOF MS as a tool to identify foodborne yeasts and yeast-like fungi |
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| المؤلفون: | UCL - SST/ELI/ELIM - Applied Microbiology, Quintilla, Raquel, Kolecka, Anna, Casaregola, Serge, Daniel, Heide-Marie, Houbraken, Jos, Kostrzewa, Markus, Boekhout, Teun, Groenewald, Marizeth |
| المصدر: | International Journal of Food Microbiology, Vol. 266, p. 109-118 (2018) |
| بيانات النشر: | Elsevier BV 2018 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Since food spoilage by yeasts causes high economic losses, fast and accurate identifications of yeasts associated with food and food-related products are important for the food industry. In this study the efficiency of the matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify food related yeasts was evaluated. A CBS in-house MALDI-TOF MS database was created and later challenged with a blinded test set of 146 yeast strains obtained from food and food related products. Ninety eight percent of the strains were correctly identified with log score values > 1.7. One strain, Mrakia frigida, gained a correct identification with a score value < 1.7. Two strains could not be identified at first as they represented a mix of two different species. These mixes were Rhodotorula babjevae with Meyerozyma caribbica and Clavispora lusitaniae with Debaryomyces hansenii. After separation, all four species could be correctly identified with scores > 1.7. Ambiguous identifications were observed due to two incorrect reference mass spectra's found in the commercial database BDAL v.4.0, namely Candida sake DSM 70763 which was re-identified as Candida oleophila, and Candida inconspicua DSM 70631 which was re-identified as Pichia membranifaciens. MALDI-TOF MS can distinguish between most of the species, but for some species complexes, such as the Kazachstania telluris and Mrakia frigida complexes, MALDI-TOF MS showed limited resolution and identification of sibling species was sometimes problematic. Despite this, we showed that the MALDI-TOF MS is applicable for routine identification and validation of foodborne yeasts, but a further update of the commercial reference databases is needed. |
| مصطلحات الفهرس: | Food Science, Microbiology, General Medicine, Food yeast, MALDI-TOF MS, Database, Taxonomic relatives, Sibling species, info:eu-repo/semantics/article |
| URL: | |
| الاتاحة: | Open access content. Open access content info:eu-repo/semantics/closedAccess |
| ملاحظة: | English |
| Other Numbers: | UCDLC oai:dial.uclouvain.be:boreal:193910 boreal:193910 info:doi/10.1016/j.ijfoodmicro.2017.11.016 urn:ISSN:0168-1605 urn:EISSN:1879-3460 1130455454 |
| المصدر المساهم: | UNIVERSITE CATHOLIQUE DE LOUVAIN From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1130455454 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |