Electronic Resource
Engineering Gram-Negative Microbial Cell Factories Using Transposon Vectors
| العنوان: | Engineering Gram-Negative Microbial Cell Factories Using Transposon Vectors |
|---|---|
| المؤلفون: | Comunidad de Madrid, Ministerio de Economía y Competitividad (España), European Commission, European Research Council, Martínez-García, Esteban, Aparicio, Tomás, Lorenzo, Víctor de, Nikel, Pablo I. |
| بيانات النشر: | Springer Nature 2016-10 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The construction of microbial cell factories à la carte largely depends on specialized molecular biology and synthetic biology tools needed to reprogram bacteria for modifying their existing functions or for bestowing them with new-to-Nature tasks. In this chapter, we document the use of a series of broad-host-range mini-Tn5 vectors for the delivery of gene(s) into the chromosome of Gram-negative bacteria and for the generation of saturated, random mutagenesis libraries for studies of gene function. The application of these tailored mini-transposon vectors, which could also be used for chromosomal engineering of a wide variety of Gram-negative microorganisms, is demonstrated in the platform environmental bacterium Pseudomonas putida KT2440. |
| مصطلحات الفهرس: | Mini-transposon, Tn5 transposon, Pseudomonas putida, Escherichia coli, Synthetic biology, Metabolic engineering, Microbial cell factory, Genome editing, capítulo de libro |
| URL: | Methods in Molecular Biology 1498 Preprint Sí info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RTC-2014-1777-3 info:eu-repo/grantAgreement/EC/FP7/289326 S2010/BMD-2414/PROMPT info:eu-repo/grantAgreement/EC/FP7/610730 info:eu-repo/grantAgreement/EC/FP7/610730 info:eu-repo/grantAgreement/EC/H2020/635536 |
| الاتاحة: | Open access content. Open access content openAccess |
| ملاحظة: | English |
| Other Numbers: | CTK oai:digital.csic.es:10261/146960 In Vitro Mutagenesis : 273-293 (2016) 978-1-4939-6470-3 10.1007/978-1-4939-6472-7_18 978-1-4939-6472-7 1105205575 |
| المصدر المساهم: | CSIC From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1105205575 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |