Electronic Resource
Fission yeast Stn1 is crucial for semi-conservative replication at telomeres and subtelomeres
| العنوان: | Fission yeast Stn1 is crucial for semi-conservative replication at telomeres and subtelomeres |
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| المؤلفون: | 70551381, 30184493, Takikawa, Masahiro, Tarumoto, Yusuke, Ishikawa, Fuyuki |
| بيانات النشر: | Oxford University Press (OUP) 2017-02-17 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The CST complex is a phylogenetically conserved protein complex consisting of CTC1/Cdc13, Stn1 and Ten1 that protects telomeres on linear chromosomes. Deletion of the fission yeast homologs stn1 and ten1 results in complete telomere loss; however, the precise function of Stn1 is still largely unknown. Here, we have isolated a high-temperature sensitive stn1 allele (termed stn1-1). stn1-1 cells abruptly lost telomeric sequence almost completely at the restrictive temperature. The loss of chromosomal DNA happened without gradual telomere shortening, and extended to 30 kb from the ends of chromosomes. We found transient and modest single-stranded G-strand exposure, but did not find any evidence of checkpoint activation in stn1-1 at the restrictive temperature. When we probed neutral-neutral 2D gels for subtelomere regions, we found no Y-arc-shaped replication intermediates in cycling cells. We conclude that the loss of telomere and subtelomere DNAs in stn1-1 cells at the restrictive temperature is caused by very frequent replication fork collapses specifically in subtelomere regions. Furthermore, we identified two independent suppressor mutants of the high-temperature sensitivity of stn1-1: a multi-copy form of pmt3 and a deletion of rif1. Collectively, we propose that fission yeast Stn1 primarily safeguards the semi-conservative DNA replication at telomeres and subtelomeres. |
| مصطلحات الفهرس: | journal article |
| URL: | 10.1093/nar/gkw1176 |
| الاتاحة: | Open access content. Open access content © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| ملاحظة: | English |
| Other Numbers: | YJ@ oai:repository.kulib.kyoto-u.ac.jp:2433/227742 1362-4962 Nucleic Acids Res 45 3 1255 1269 28180297 1024078266 |
| المصدر المساهم: | KYOTO UNIV From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1024078266 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |