Electronic Resource
Enzyme Nicotinamide Cofactor Specificity Reversal Guided by Automated Structural Analysis and Library Design
| العنوان: | Enzyme Nicotinamide Cofactor Specificity Reversal Guided by Automated Structural Analysis and Library Design |
|---|---|
| المؤلفون: | Jensen, Michael Krogh, Keasling, Jay D., Cahn, Jackson K. B., Brinkmann-Chen, Sabine, Arnold, Frances H. |
| بيانات النشر: | Humana Press 2017-11-24 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The specificity of enzymes for nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP) as redox carriers can pose a significant hurdle for metabolic engineering and synthetic biology applications, where switching the specificity might be beneficial. We have developed an easy-to-use computational tool (CSR-SALAD) for the design of mutant libraries to simplify the process of reversing the cofactor specificity of an enzyme. Here, we describe the optimal use of this tool and present methods for its application in a laboratory setting. |
| مصطلحات الفهرس: | Book Section, PeerReviewed |
| URL: | CaltechAUTHORS:20171127-143418550 10.1007/978-1-4939-7295-1_2 |
| الاتاحة: | Open access content. Open access content |
| ملاحظة: | Enzyme Nicotinamide Cofactor Specificity Reversal Guided by Automated Structural Analysis and Library Design |
| Other Numbers: | CIT oai:authors.library.caltech.edu:83459 Cahn, Jackson K. B. and Brinkmann-Chen, Sabine and Arnold, Frances H. (2017) Enzyme Nicotinamide Cofactor Specificity Reversal Guided by Automated Structural Analysis and Library Design. In: Synthetic Metabolic Pathways. Methods in Molecular Biology. No.1671. Humana Press , New York, NY, pp. 15-26. ISBN 978-1-4939-7294-4. https://resolver.caltech.edu/CaltechAUTHORS:20171127-143418550 <https://resolver.caltech.edu/CaltechAUTHORS:20171127-143418550> 1017652296 |
| المصدر المساهم: | CALIFORNIA INST OF TECH From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1017652296 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |