Electronic Resource
Immunorecognition magnetic supports for the development of an electrochemical immunoassay for azaspiracid detection in mussels
| العنوان: | Immunorecognition magnetic supports for the development of an electrochemical immunoassay for azaspiracid detection in mussels |
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| المؤلفون: | Group of Nanobiotechnology and Bioanalysis, Departament d'Enginyeria Química, Universitat Rovira i Virgili, O'Sullivan, C.K.; Leonardo, S.; Rambla-Alegre, M.; Samdal, I.A.; Miles, C.O.; Kilcoyne, J.; Diogène, J.; Campàs, M. |
| المصدر: | Biosensors & Bioelectronics |
| بيانات النشر: | 2017 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Immunorecognition magnetic supports for the development of an electrochemical immunoassay for azaspiracid detection in mussels DOI: 10.1016/j.bios.2017.02.015 URL: http://www.sciencedirect.com/science/article/pii/S0956566317301021 Filiació URV: SI Memòria As azaspiracids (AZAs) are being reported from the coastal waters of an increasing number of countries on a global scale, the need for rapid, simple and cost-effective methods to detect these marine toxins and protect seafood consumers’ health is becoming evident. A magnetic bead (MB)-based direct immunoassay for the detection of AZAs, using protein G-coated MBs as supports for antibody immobilisation and peroxidase-labelled AZA as a tracer is detailed. A colorimetric approach was first developed to optimise the experimental parameters and establish the cross-reactivity factors for AZA-1–10. The subsequent combination of the immunorecognition MBs with 8-electrode arrays enabled the multiplexed electrochemical detection of AZAs. Naturally-contaminated mussel samples were analysed and the results obtained showed an excellent correlation with LC-MS/MS analysis. The MB-based immunoassay facilitated the quantification of a wide range of AZA concentrations (120–2875 μg AZA-1 equiv./kg), with a limit of detection (63 μg AZA-1 equiv./kg) below the European regulatory threshold, using a protocol that requires very few steps and a short analysis time (~ 15 min). The simplicity, cost-effectiveness, rapidity, robustness, selectivity and precision of the assay provide a valuable tool for the detection of all regulated AZAs and other toxic AZA analogues, suitable for end users in the field of food safety. |
| مصطلحات الفهرس: | Musclos, Immunologia, Toxines, Horseradish Peroxidase, electrochemical, Azaspiracid, Biochemistry and technology, Bioquímica y tecnología, Bioquímica i biotecnologia, 0956-5663, article, Article, Artículo |
| URL: | |
| الاتاحة: | Open access content. Open access content closedAccess |
| ملاحظة: | Anglès 525 kb |
| Other Numbers: | RIV oai:urv.cat:PC:2589 1012905690 |
| المصدر المساهم: | UNIVERSITAT ROVIRA I VIRGILI BIBLIOTECA From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1012905690 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |