Electronic Resource
Adenovirus-mediated TA-p73?? gene transfer increases chemosensitivity of human malignant melanomas
| العنوان: | Adenovirus-mediated TA-p73?? gene transfer increases chemosensitivity of human malignant melanomas |
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| المؤلفون: | Department of Dermatology, University of Michigan Comprehensive Cancer Center, Ann Arbor, MI, 28109, USA, Department of Vectorology and Experimental Gene Therapy, University of Rostock, Schillingallee 70, Rostock, 18055, Germany, Department of Vectorology and Experimental Gene Therapy, University of Rostock, Schillingallee 70, Rostock, 18055, Germany; Department of Vectorology and Experimental Gene Therapy, University of Rostock, Schillingallee 70, Rostock, D-18055, Germany, Ann Arbor, Tuve, S., Racek, T., Niemetz, A., Schultz, J., Soengas, M.S., P??tzer, B.M. |
| بيانات النشر: | Kluwer Academic Publishers; Springer Science + Business Media, Inc. 2006-09-11T14:41:41Z 2006-09-11T14:41:41Z 2006-02 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Malignant melanoma is the most aggressive form of skin cancer and has proven to be highly resistant to conventional chemotherapy. Intriguingly, the p53 tumor suppressor, a main mediator of chemoresistance in other tumor types, is rarely mutated in melanoma. However, we have previously shown that anti-apoptotic isoforms of p73 (??TA-p73), another member of the p53 family, are overexpressed in metastatic melanomas.??TA-p73 can oppose the pro-apoptotic functions of p53 and full length p73, and thus it could contribute to melanoma chemoresistance. In this study, we use an efficient adenoviral-based gene transfer approach to introduce a transcriptionally active form of p73 (TA-p73??) in melanoma cells, with the objective of overcoming drug resistance. Interestingly, TA-p73?? significantly sensitized 5 out of 7 aggressive melanoma cell lines to the standard therapeutic agents adriamycin and cisplatin. More importantly, TA-p73?? displayed a synergistic effect in vivo allowing adriamycin or cisplatin to block melanoma cell growth in mouse xenograft models (p <0.05). In summary, our data show that Ad-mediated TA-p73?? gene expression can markedly sensitize a subset of melanoma cell lines to adriamycin and cisplatin in vitro and in vivo, suggesting a new chemosensitization strategy for malignant melanomas. |
| URL: | |
| الاتاحة: | Open access content. Open access content |
| ملاحظة: | En_US |
| Other Numbers: | EYM oai:deepblue.lib.umich.edu:2027.42/44369 1360-8185 1573-675X 16502261 Apoptosis 894072614 |
| المصدر المساهم: | From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.ocn894072614 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |