Electronic Resource
Labeling and exocytosis of secretory compartments in RBL mastocytes by polystyrene and mesoporous silica nanoparticles
| العنوان: | Labeling and exocytosis of secretory compartments in RBL mastocytes by polystyrene and mesoporous silica nanoparticles |
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| المؤلفون: | Ekkapongpisit,Maneerat, Giovia ,Antonino, Nicotra,Giuseppina, Ozzano ,Matteo, Caputo,Giuseppe, Isidoro,Ciro |
| بيانات النشر: | Dove Press 2012-04-04 |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Maneerat Ekkapongpisit1,*, Antonino Giovia1,*, Giuseppina Nicotra1, Matteo Ozzano1, Giuseppe Caputo2,3, Ciro Isidoro1 1Laboratory of Molecular Pathology and Nanobioimaging, Department of Health Sciences, Università del Piemonte Orientale "A. Avogadro", Novara, Italy; 2Department of Chemistry, University of Turin, Turin, 3Cyanine Technology SpA, Torino, Italy *These authors contributed equally to this workBackground: For a safe ‘in vivo’ biomedical utilization of nanoparticles, it is essential to assess not only biocompatibility, but also the potential to trigger unwanted side effects at both cellular and tissue levels. Mastocytes (cells having secretory granules containing cytokines, vasoactive amine, and proteases) play a pivotal role in the immune and inflammatory responses against exogenous toxins. Mastocytes are also recruited in the tumor stroma and are involved in tumor vascularization and growth.Aim and methods: In this work, mastocyte-like rat basophilic leukemia (RBL) cells were used to investigate whether carboxyl-modified 30 nm polystyrene (PS) nanoparticles (NPs) and naked mesoporous silica (MPS) 10 nm NPs are able to label the secretory inflammatory granules, and possibly induce exocytosis of these granules. Uptake, cellular retention and localization of fluorescent NPs were analyzed by cytofluorometry and microscope imaging.Results: Our findings were that: (1) secretory granules of mastocytes are accessible by NPs via endocytosis; (2) PS and MPS silica NPs label two distinct subpopulations of inflammatory granules in RBL mastocytes; and (3) PS NPs induce calcium-dependent exocytosis of inflammatory granules.Conclusion: These findings highlight the value of NPs for live imaging of inflammatory processes, and also have important implications for the clinical use of PS-based NPs, due to their potential to trigger the unwanted activation of mastocytes.Keywords: secretory lysosomes, inflammation, nanoparticle |
| مصطلحات الفهرس: | International Journal of Nanomedicine, Original Research, info:eu-repo/semantics/article |
| URL: | info:eu-repo/semantics/altIdentifier/doi/10.2147/IJN.S29034 |
| الاتاحة: | Open access content. Open access content info:eu-repo/semantics/openAccess |
| ملاحظة: | text/html English |
| Other Numbers: | NZDMP oai:dovepress.com/9632 https://www.dovepress.com/labeling-and-exocytosis-of-secretory-compartments-in-rbl-mastocytes-by-peer-reviewed-fulltext-article-IJN 805759689 |
| المصدر المساهم: | DOVE MEDL PRESS LTD From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.ocn805759689 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |