التفاصيل البيبلوغرافية
| العنوان: |
Vliv nového kryokonzervačního protokolu na imunogenicitu a rejekci tepenných aloštěpů u potkanů |
| Alternate Title: |
Influence of new cryopreservation protocol on immunogenicity and rejection of arterial allografts in rats |
| المؤلفون: |
Hrubý, Jan |
| Thesis Advisors: |
Matia, Ivan, Oliverius, Martin, Štádler, Petr |
| سنة النشر: |
2021 |
| المجموعة: |
Czech ETDs |
| مصطلحات موضوعية: |
myointimální hyperplázie, akutní rejekce, kryokonzervace, acute rejection, myointimal hyperplasia, MHC protilátky třídy I a II, cryopreservation, alogenní cévní štěp, isogenní, allogenic vascular graft, MHC class I and II antibodies, isogenic |
| الوصف: |
The aim of the presented experimental work was to study an acute cell and antibody- mediated immune response in recipients of abdominal aortic grafts treated by a new standardized clinical cryopreservation/slow thawing protocol used in the "Vascular graft transplant program in the Czech Republic" in a rat model. Another aim of our study was to compare the influence of two basic types of conservation protocols used in this program (cryopreservation/slow thawing protocol and cold-stored protocol) on the acute immune response after transplantation of such treated abdominal aortic grafts in rats. Cryopreserved abdominal aortic grafts were transplanted syngeneously between Lewis rats (CRYO-ISO group, cryopreservation period 172.6 days) and allogeneically between Brown-Norway and Lewis rats (CRYO-ALO group, cryopreservation period 179.3 days). The grafts were explanted on day 30 after transplantation and subsequently examined by histological and immunohistochemical methods, focusing on typical signs of acute rejection in the three basic layers of the aortic wall. We monitored the presence of endothelial cells, signs of intimal hyperplasia, tunica media thickness, the presence of necrosis and deposition of imunoglobulin class G in this layer, the number of CD4+, CD8+ and LEW MHC II+ immunocompetent cells... |
| Original Identifier: |
oai:invenio.nusl.cz:447268 |
| نوع الوثيقة: |
Doctoral Thesis |
| اللغة: |
Czech |
| الاتاحة: |
http://www.nusl.cz/ntk/nusl-447268 |
| Rights: |
info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: |
edsndl.nusl.cz.oai.invenio.nusl.cz.447268 |
| قاعدة البيانات: |
Networked Digital Library of Theses & Dissertations |