التفاصيل البيبلوغرافية
| العنوان: |
Functional variation in the hypoxia-inducible factor (HIF) pathway in humans |
| المؤلفون: |
Petousi, Nayia |
| Thesis Advisors: |
Robbins, Peter A.; Ratcliffe, Peter J. |
| بيانات النشر: |
University of Oxford, 2012. |
| سنة النشر: |
2012 |
| المجموعة: |
Ethos UK |
| Original Material: |
http://ora.ox.ac.uk/objects/uuid:bf340955-5125-422e-befb-b2a7d55a1d39 |
| مصطلحات موضوعية: |
612.22, Respiratory medicine, Medical Sciences, Gene medicine, Biology (medical sciences), Genetics (medical sciences), Physiology, hypoxia, hypoxia-inducible factor (HIF), Tibetan, cardiorespiratory physiology, evolution |
| الوصف: |
By undertaking a number of different experimental approaches at the genetic, cellular/ molecular and integrative physiology levels, I investigated functional variation in the Hypoxia-Inducible Factor (HIF) transcription pathway in humans. My studies focused on Tibetan natives. Tibetan highlanders are adapted to life in a hypoxic environment and exhibit distinct physiological traits at high altitude. Recent studies identified positive selection at two genetic loci, EPAS1 (HIF2α) and EGLN1 (PHD2), in Tibetan highlanders and demonstrated an association of EGLN1/EPAS1 genotype with haemoglobin concentration. Both are genes of the HIF pathway, which coordinates an organism’s response to hypoxia. Patients living at sea level with genetic diseases of the HIF pathway have characteristic phenotypes at both the integrative physiology and cellular levels. I investigated whether Tibetans living at sea level also possess distinct phenotypic characteristics, and whether these may be related to underlying variation within the HIF pathway. I compared Tibetans living at sea level with Han Chinese, their most closely-related major ethnic group, and found that Tibetans possess a significantly different integrative physiology phenotype. Tibetans had a lower haemoglobin concentration and haematocrit, a higher pulmonary ventilation relative to metabolism, and blunted pulmonary vascular responses to both acute (minutes) and sustained (8 hours) hypoxia. Regarding genotype- phenotype relationships within the Tibetans, I found a significant correlation between both EPAS1 and EGLN1 genotype and the induction of erythropoietin by systemic hypoxia. At an intermediate cellular level, the relative expression and the hypoxic induction of HIF- regulated genes were significantly lower in peripheral blood lymphocytes from Tibetans compared with Han Chinese. I also investigated whether the genetic variation in EPAS1 selected for in Tibetans may be functional at the molecular level by affecting transcription of EPAS1 in cells and whether certain coding variants in EGLN1 found in Tibetans affect protein (PHD2) activity in cells and in vitro. A small supplementary study was undertaken in patients with idiopathic erythrocytosis, who have elevated or inappropriately normal erythropoietin levels, to investigate if they have genetic alterations in the HIF system. |
| Original Identifier: |
oai:ethos.bl.uk:581178 |
| نوع الوثيقة: |
Electronic Thesis or Dissertation |
| الاتاحة: |
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.581178 |
| رقم الانضمام: |
edsndl.bl.uk.oai.ethos.bl.uk.581178 |
| قاعدة البيانات: |
Networked Digital Library of Theses & Dissertations |