Academic Journal
Alcoholic Fractions F5 and F6 from Withania somnifera Leaves Show a Potent Antileishmanial and Immunomodulatory Activities to Control Experimental Visceral Leishmaniasis
| العنوان: | Alcoholic Fractions F5 and F6 from Withania somnifera Leaves Show a Potent Antileishmanial and Immunomodulatory Activities to Control Experimental Visceral Leishmaniasis |
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| المؤلفون: | Chandrasekaran, Sambamurthy, Veronica, Jalaja, Sundar, Shyam, Maurya, Radheshyam |
| Thesis Advisors: | Univ Arizona, Inst BIO5 |
| بيانات النشر: | FRONTIERS MEDIA SA, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | University of Arizona |
| Original Material: | Alcoholic Fractions F5 and F6 from Withania somnifera Leaves Show a Potent Antileishmanial and Immunomodulatory Activities to Control Experimental Visceral Leishmaniasis 2017, 4 Frontiers in Medicine Frontiers in Medicine |
| مصطلحات موضوعية: | visceral leishmaniasis, peritoneal mouse macrophages, Withania somnifera, withaferin-A, IL-10 |
| الوصف: | Visceral leishmaniasis (VL) causes fatal life-threatening disease, if left untreated. The current drugs have various limitations; hence, natural products from medicinal plants are being focused in search of new drugs to treat leishmaniasis. The aim of the present study was to evaluate the antileishmanial and immunomodulatory activities of F5 and F6 alcoholic fractions from Withania somnifera leaves and purified withaferin-A in Leishmania donovani-infected peritoneal macrophages and BALB/c mice. We observed that F5 (15 mu g/mL), F6 (10 mu g/mL), and withaferin-A (1.5 mu M) reduce amastigote count in peritoneal macrophages and induce reactive oxygen species and significant decrease in IL-10 mRNA expression compared to control upon treatment. Subsequently, in vivo study mice were treated with F5 (25 and 50 mg/kg b.wt.), F6 (25 and 50 mg/kg b.wt.) orally, and withaferin-A (2 mg/kg b.wt.) intraperitoneally for 10 consecutive days and a drastic reduction in parasite burden in both spleen and liver were observed. The treatment resulted in the reduction in IL-10, IL-4, and TGF-beta mRNA expression and a significant increase in IFN-gamma /IL-10 expression ratio in the treated group compared to control. The humoral response of these alcoholic fractions and withaferin-A shows increased IgG2a levels when compared with IgG1 in treated mice. Taken together, our result concludes that withanolides in alcoholic fractions demonstrate a potent antileishmanial and immunomodulatory activities in experimental VL. |
| Original Identifier: | oai:arizona.openrepository.com:10150/625511 |
| نوع الوثيقة: | Article |
| اللغة: | English |
| تدمد: | 2296-858X |
| Relation: | http://journal.frontiersin.org/article/10.3389/fmed.2017.00055/full |
| DOI: | 10.3389/fmed.2017.00055 |
| الاتاحة: | http://hdl.handle.net/10150/625511 http://arizona.openrepository.com/arizona/handle/10150/625511 |
| Rights: | © 2017 Chandrasekaran, Veronica, Sundar and Maurya. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). |
| رقم الانضمام: | edsndl.arizona.edu.oai.arizona.openrepository.com.10150.625511 |
| قاعدة البيانات: | Networked Digital Library of Theses & Dissertations |
Full Text Finder | تدمد: | 2296858X |
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| DOI: | 10.3389/fmed.2017.00055 |