Dissertation/ Thesis
Dynamic Study based on Surface-Enhanced Infrared Spectroscopy for Self-Assembled Monolayers Modified Process
العنوان: | Dynamic Study based on Surface-Enhanced Infrared Spectroscopy for Self-Assembled Monolayers Modified Process |
---|---|
Alternate Title: | 應用表面增強紅外光技術於自組性單分子薄膜修飾的動態探討 |
المؤلفون: | Yu-ChenChang, 張祐誠 |
Thesis Advisors: | Hsien-Chang Chang, 張憲彰 |
سنة النشر: | 2014 |
المجموعة: | National Digital Library of Theses and Dissertations in Taiwan |
الوصف: | 102 With the development of biotechnology, the detection of macromolecules needs to fabricate a bio-affinity interface to capture or react with the target species. It should be noted that the fabrication of these modified layer often depended on the experience of researchers, and the characteristic of these biosensors were still trial and error. A novel technique is desired to investigate the structure and adsorbed orientation of these layers. ATR-SEIRAS has the advantages of high sensitivity, nondestructive test for biological molecules, nanoscale range of interface signals detection and high spatio-temporal resolution. In this study, The study is interested in the SAMs modification on gold surface. It made time-resolved SEIRAS to investigate the modification of 11-MUA SAMs in ethanol, the activation of EDC and NHSS in MES buffer, and covalent attachment of amide bonds in HEPES buffer, which can be clearly discussed to provide fundamental information for further biosensor application. The chemical absorption of 11-MUA SAMs completed within a few minutes. However, the regular arrangement of the hydrocarbon backbone is related to coverage, which lead to the modified time. The peak of the asymmetric stretch of COO can be used to evaluate the modified time is around 4 hours. The activation time is also done by the stretch of C=O of NHSS activate ester, which for 10 mM EDC and 2 mM NHS is about 50 minutes. Finally, The modification of histidine and histamine are also be investigated. |
Original Identifier: | 102NCKU5159190 |
نوع الوثيقة: | 學位論文 ; thesis |
وصف الملف: | 52 |
الاتاحة: | http://ndltd.ncl.edu.tw/handle/3jt8dn |
رقم الانضمام: | edsndl.TW.102NCKU5159190 |
قاعدة البيانات: | Networked Digital Library of Theses & Dissertations |
الوصف غير متاح. |