Dissertation/ Thesis
Production of Fructosyltransferase by Solid State Fermentation of Aspergillus japonicus
| العنوان: | Production of Fructosyltransferase by Solid State Fermentation of Aspergillus japonicus |
|---|---|
| Alternate Title: | 利用日本麴菌的固態醱酵生產果糖轉移酶 |
| المؤلفون: | Kuo-Chen Wu, 吳國珍 |
| Thesis Advisors: | Dey-Chyi Sheu, 許垤棊 |
| سنة النشر: | 2008 |
| المجموعة: | National Digital Library of Theses and Dissertations in Taiwan |
| الوصف: | 96 Production of fructosyltransferase by solid state culture of Aspergillus japonicus was investigated. The fermentation was carried out in 500-mL Erlenmeyer flask at 30°C after an inoculation of spore suspension. The culture medium was a mixture of 20 g wheat bran, 10 g rice hull, 10 mL of 10 % (w/v) yeast extract, 10 mL of 50 % (w/v) sucrose and 5 mL of 0.065 M potassium phosphate buffer (pH 7). During a 7-day incubation, maximum enzyme activity up to 113102 U/flask was achieved at day 6. Production of fructosyltransferase was affected by the morphology of the fungus. The enzyme activity increased as white vegetative mycelia increased. However, the enzyme decayed when the color of the culture was converting to black due to the production of spores. Production of fructosyltransferase increased up to 117851 U/flask when the 50 % (w/v) sucrose was replaced by 60 % (w/v) sucrose. However more sucrose (i.e. 70 %,w/v) did not result more enzyme production. |
| Original Identifier: | 096TTU05106005 |
| نوع الوثيقة: | 學位論文 ; thesis |
| وصف الملف: | 64 |
| الاتاحة: | http://ndltd.ncl.edu.tw/handle/10503387497765418380 |
| رقم الانضمام: | edsndl.TW.096TTU05106005 |
| قاعدة البيانات: | Networked Digital Library of Theses & Dissertations |
| الوصف غير متاح. |