Dissertation/ Thesis
Combining Radiotherapy and Adv-IL3 Gene Therapy for Prostate Cancer Therapy
| العنوان: | Combining Radiotherapy and Adv-IL3 Gene Therapy for Prostate Cancer Therapy |
|---|---|
| Alternate Title: | 結合放射治療與腺病毒-細胞激素3基因治療以治療攝護腺癌 |
| المؤلفون: | Chen-lin Hsieh, 謝承霖 |
| Thesis Advisors: | Chi-shiun Chiang, 江啟勳 |
| سنة النشر: | 2003 |
| المجموعة: | National Digital Library of Theses and Dissertations in Taiwan |
| الوصف: | 91 The aim of this research is to combine radiation therapy and adenovirus-IL3 gene therapy for TRAMP C-1 prostate cancer. Interleukin 3 (IL-3) is known as a hematopoietic factor. Its function is not only to stimulate stem cell proliferation and differentiation, but is also able to stimulate cytotoxic T cell recognizing and killing cancer cells in the model of cancer immunogene therapy. Adenovirus vector is a useful vector to deliver genes into cancer cells in situ and is more practical for clinical application. This study showed that there was not significant difference between the group of combining radiation therapy and adenovirus-IL3 gene therapy and the group of radiation therapy only. The results of RT-PCR suggested that adenovirus vector induced stronger host immune response than IL-3 gene therapy. The choice of other vectors that do not elicit host immune response is suggested. The second topic of this research is to study the histological changes and the pattern of gene expression profile in TRAMP mice (transgenic adenocarcinoma of the mice prostate) at different ages. RT-PCR results showed that both prostate and lung tissues expressed SV 40-Tag oncogene and probasin mRNAs. The mSTEAP gene should be expressed only in prostate, kidney and testis, but its expression was also found in lung tissue. mTEM1 gene should be expressed and up-regulated only in angiogenesis tumors. However, the result showed that mTEM1 was also highly expressed in lung tissue in TRAMP mice. |
| Original Identifier: | 091NTHU0257032 |
| نوع الوثيقة: | 學位論文 ; thesis |
| وصف الملف: | 77 |
| الاتاحة: | http://ndltd.ncl.edu.tw/handle/03342777527597461002 |
| رقم الانضمام: | edsndl.TW.091NTHU0257032 |
| قاعدة البيانات: | Networked Digital Library of Theses & Dissertations |
| الوصف غير متاح. |