Dissertation/ Thesis
Cloning and characterization of the flgM and flgN genes from Salmonella choleraesuis
| العنوان: | Cloning and characterization of the flgM and flgN genes from Salmonella choleraesuis |
|---|---|
| Alternate Title: | 豬霍亂沙門氏桿菌flgM與flgN基因的選殖及其功能的探討 |
| المؤلفون: | Ming-Yi Li, 李明怡 |
| Thesis Advisors: | Kuo-Chieh Ho, Gan-Nan Chang, 何國傑, 張甘楠 |
| سنة النشر: | 2002 |
| المجموعة: | National Digital Library of Theses and Dissertations in Taiwan |
| الوصف: | 90 Salmonella choleraesuis is a host adapted, facultative intracellular pathogen and belongs to the family Enterobacteriaceae. It is a pathogen of human and pigs, more serious in pigs. It causes swine septicemia, enterocolitis or bacteremic localization as pneumonia, or occasionally as meningitis or encephalitis. This study is focused on flgM and flgN of Salmonella choleraesuis. These two genes is responsible for filament formation. Filament is one part of flagellum. For investigating the function of flgM and flgN, the genes were cloned from Salmonella choleraesuis. A knockout strain that flgM and flgN was inactivated by inserting an Ω fragment into the genes. Then vector containing normal flgM, flgN or flgM and flgN was introduced into the knockout strain respectively. The data showed that when flgM and flgN was inactivated, the number of flagella were fewer and motility were lawer than normal bacteria. When normal flgM and flgN was re-introduced into knockout strain, the number of flagella and motility were recovered, but not the same as normal bacteria. The results were the same when normal flgM was re-introduced into knockout strain. However, the normal flgN could not restore the number of flagella and the motility of knockout strain. The results suggested that flgM and flgN could interact in some way to affect the formation of flagella, not by single function of flgM or flgN. |
| Original Identifier: | 090NTU00366001 |
| نوع الوثيقة: | 學位論文 ; thesis |
| وصف الملف: | 52 |
| الاتاحة: | http://ndltd.ncl.edu.tw/handle/54228171678021395178 |
| رقم الانضمام: | edsndl.TW.090NTU00366001 |
| قاعدة البيانات: | Networked Digital Library of Theses & Dissertations |
| الوصف غير متاح. |