Academic Journal
UVA/Riboflavin-Induced Apoptosis in Mouse Cornea
| العنوان: | UVA/Riboflavin-Induced Apoptosis in Mouse Cornea |
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| المؤلفون: | Wang, Fan |
| المصدر: | Ophthalmologica 2008;222:369–372, ISSN: 0030-3755 |
| بيانات النشر: | Karger, 2014. |
| سنة النشر: | 2014 |
| المجموعة: | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Original Material: | urn:nbn:de:bsz:14-qucosa-133626 409466425 |
| مصطلحات موضوعية: | info:eu-repo/classification/ddc/610, ddc:610, UV-Bestrahlung, Riboflavin, Vorbehandlung, antigenpräsentierende Zellen, Kornea, Hornhaut, Apoptose, Ultraviolet irradiation, Riboflavin pretreatment, Antigen-presenting cells, Cornea, Apoptosis |
| الوصف: | Background: A mouse model of combined UVA/riboflavin irradiation to eliminate stromal cells and other antigen-presenting cells in the cornea provides the basis for a probably low risk of corneal transplantation. Methods: After abrasion of the epithelium, the central corneas of mouse eyes were treated with UVA/riboflavin in vitro. Histological studies of hematoxylin-eosin and immunohistochemical staining with caspase 3 were performed. Dissected mouse corneas were analyzed by Western blot. Results: Apoptotic cells were shown on the central corneal stroma; a cell-free zone was displayed in the cornea. Numbers of dead cells increased according to cultivation time. However, the endothelium survived due to the adjustment of the irradiation dose. Conclusions: A cell-free zone in the stroma of the mouse cornea was produced by UVA/riboflavin irradiation in vitro. The technique makes possible to prevent or reduce immunological reactions and the risk of graft rejection by pretreatment of the donor cornea, ultimately prolonging graft survival. Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich. |
| Original Identifier: | oai:qucosa:de:qucosa:27521 |
| نوع الوثيقة: | Article |
| اللغة: | English |
| DOI: | 10.1159/000151247 |
| الاتاحة: | https://tud.qucosa.de/id/qucosa%3A27521 https://tud.qucosa.de/api/qucosa%3A27521/attachment/ATT-0/ |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsndl.DRESDEN.oai.qucosa.de.qucosa.27521 |
| قاعدة البيانات: | Networked Digital Library of Theses & Dissertations |
| DOI: | 10.1159/000151247 |
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