Academic Journal
Partial purification and characterization of a thermostable alkaline protease from Lactobacillus brevis
| العنوان: | Partial purification and characterization of a thermostable alkaline protease from Lactobacillus brevis |
|---|---|
| المؤلفون: | Femi-Ola, T.O., Oladokun, D.O. |
| المصدر: | Malaysian Journal of Microbiology, Vol 8, Iss 1, Pp 1-5 (2012) |
| بيانات النشر: | Malaysian Society for Microbiology, 2012. |
| سنة النشر: | 2012 |
| المجموعة: | LCC:Microbiology |
| مصطلحات موضوعية: | Protease, Lactobacillus, Alkaline, Inhibitors, Detergent, Microbiology, QR1-502 |
| الوصف: | Aims: The research was done to study the partial purification and characterization of thermostable alkaline protease from Lactobacillus brevis.Methodology and Results: The enzyme was purified in a two-step procedure involving ammonium sulphate precipitation and Sephadex G-150 gel permeation chromatography. The protease was purified 8.04 fold with a yield of approximately 30% after purification with Sephadex G-150 column. It has a relative molecular weight of 33.2 kDa and optimally active at a temperature of 60 oC and pH 9.0. The maximum velocity Vmax and Michaelis constant Km of the protease produced during the hydrolysis of casein were 66.66 U/mg protein and 3.33 mg/ml. It was strongly activated by Ca2+ and ethylene diamine tetra acetic acid (EDTA), mildly inhibited by Na+, K+, Mg2+ and Fe2+ and strongly inhibited by Cu2+ and Hg2+. The ability of the enzyme to improve the cleansing power of various detergents was also studied. Conclusion, significance and impact of study: The findings in this study suggest that the protease is a suitable candidate for detergent formulation and biotechnological applications. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1823-8262 2231-7538 |
| Relation: | http://web.usm.my/mjm/issues/vol8no1/Research%201.pdf; https://doaj.org/toc/1823-8262; https://doaj.org/toc/2231-7538 |
| URL الوصول: | https://doaj.org/article/fea1472d448a4bc8be89db50f918054a |
| رقم الانضمام: | edsdoj.fea1472d448a4bc8be89db50f918054a |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 18238262 22317538 |
|---|