التفاصيل البيبلوغرافية
| العنوان: |
Evaluation of sample treatments in a safe and straightforward procedure for the detection of SARS-CoV-2 in saliva |
| المؤلفون: |
Camila P. Rubio, Lorena Franco-Martínez, Cristina Sánchez Resalt, Alberto Torres-Cantero, Irene Martinez-Morata, Enrique Bernal, María J. Alcaraz, María R. Vicente-Romero, Silvia Martínez-Subiela, Asta Tvarijonaviciute, José J. Cerón |
| المصدر: |
International Journal of Infectious Diseases, Vol 108, Iss , Pp 413-418 (2021) |
| بيانات النشر: |
Elsevier, 2021. |
| سنة النشر: |
2021 |
| المجموعة: |
LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: |
SARS-CoV-2, COVID-19, Saliva, RT-LAMP, Assay, Infectious and parasitic diseases, RC109-216 |
| الوصف: |
Objectives: To evaluate four sample treatments in a safe and straightforward procedure to detect SARS-CoV-2 in saliva. Methods: Four sample treatments were evaluated in a 3-step procedure to detect SARS-CoV-2 in saliva: 1) heating at 95 °C for 5 min for sample inactivation; 2) sample treatment; 3) analysis by reverse-transcription loop-mediated isothermal amplification (LAMP). Saliva samples used were from infected individuals or were spiked with known quantities of viral particles. Results: Three treatments had a limit of detection (LOD) of 500.000 viral particles per ml of saliva and could be used to detect individuals with potential to transmit the disease. The treatment of phosphate buffer, dithiothreitol, ethylenediaminetetraacetic acid and proteinase K, with an additional 95 °C heating step, yielded a lower LOD of 95; its sensitivity ranged from 100% in patients with nasopharyngeal swab reverse-transcriptase polymerase chain reaction cycle threshold values 30. Conclusions: This report highlights the importance of an adequate sample treatment for saliva to detect SARS-CoV-2 and describes a flexible procedure that can be adapted to point-of-care. Although its sensitivity when LAMP is used is lower than reverse-transcriptase polymerase chain reaction, this procedure can contribute to COVID-19 control by detecting individuals able to transmit the disease. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1201-9712 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S1201971221004550; https://doaj.org/toc/1201-9712 |
| DOI: |
10.1016/j.ijid.2021.05.053 |
| URL الوصول: |
https://doaj.org/article/cfe93e5d2023487d80412dd57b2df741 |
| رقم الانضمام: |
edsdoj.fe93e5d2023487d80412dd57b2df741 |
| قاعدة البيانات: |
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