التفاصيل البيبلوغرافية
| العنوان: |
A non-human primate derived anti-P-selectin glycoprotein ligand-1 antibody curtails acute pancreatitis by alleviating the inflammatory responses |
| المؤلفون: |
Yuhan Li, Xiangqing Ding, Xianxian Wu, Longfei Ding, Yuhui Yang, Xiaoliang Jiang, Xing Liu, Xu Zhang, Jianrong Su, Jianqing Xu, Zhiwei Yang |
| المصدر: |
Acta Pharmaceutica Sinica B, Vol 13, Iss 11, Pp 4461-4476 (2023) |
| بيانات النشر: |
Elsevier, 2023. |
| سنة النشر: |
2023 |
| المجموعة: |
LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: |
Acute pancreatitis, PSGL-1, Non-human primate, Monoclonal antibody, Therapeutic antibody RH001-6, Adhesion of leukocytes to endothelial cells, Therapeutics. Pharmacology, RM1-950 |
| الوصف: |
Acute pancreatitis (AP) is a devastating disease characterized by an inflammatory disorder of the pancreas. P-selectin glycoprotein ligand-1 (PSGL-1) plays a crucial role in the initial steps of the adhesive at process to inflammatory sites, blockade of PSGL-1 might confer potent anti-inflammatory effects. In this study, we generated two non-human primate derived monoclonal antibodies capable of efficiently targeting human PSGL-1, RH001-6 and RH001-22, which were screened from immunized rhesus macaques. We found that RH001-6, can effectively block the binding of P-selectin to PSGL-1, and abolish the adhesion of leukocytes to endothelial cells in vitro. In vivo, we verified that RH001-6 relieved inflammatory responses and pancreatic injury in both caerulein and l-arginine induced AP models. We also evaluated the safety profile after RH001-6 treatment in mice, and verified that RH001-6 did not cause any significant pathological damages in vivo. Taken together, we developed a novel non-human primate derived PSGL-1 blocking antibody with high-specificity, named RH001-6, which can interrupt the binding of PSGL-1 and P-selectin and attenuate inflammatory responses during AP. Therefore, RH001-6 is highly potential to be further developed into therapeutics against acute inflammatory diseases, such as AP. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2211-3835 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2211383523002903; https://doaj.org/toc/2211-3835 |
| DOI: |
10.1016/j.apsb.2023.07.028 |
| URL الوصول: |
https://doaj.org/article/fd8b87e6c0f5485d83787337d19a5afc |
| رقم الانضمام: |
edsdoj.fd8b87e6c0f5485d83787337d19a5afc |
| قاعدة البيانات: |
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