Academic Journal
Effect of DMSO combined with trehalose on cryopreservation of goose semen
| العنوان: | Effect of DMSO combined with trehalose on cryopreservation of goose semen |
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| المؤلفون: | Jinhong Fu, Jingyun Ma, Ziqiang Feng, Yupu Song, Ichraf Mabrouk, Yuxuan Zhou, Yudong Wang, Xianou Fu, Honglei Jin, Yuxin Zhang, Yongfeng Sun |
| المصدر: | Journal of Applied Animal Research, Vol 51, Iss 1, Pp 84-91 (2023) |
| بيانات النشر: | Taylor & Francis Group, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Veterinary medicine |
| مصطلحات موضوعية: | DMSO, trehalose, goose, semen cryopreservation, Veterinary medicine, SF600-1100 |
| الوصف: | Poultry semen cryopreservation technology is one of the most direct methods to protect poultry germplasm, and in this study, the back and abdomen massage method is used to collect the semen of 20 Huoyan male geese, which is on the basis of EK diluent adding different combinations of dimethyl sulfoxide (DMSO) and trehalose, in total 7 different combinations treatments were achieved: D1T0 (5%DMSO), D1T1 (5% DMSO + 50 mM trehalose), D1T2 (5%DMSO + 100 mM trehalose), D2T0 (10% DMSO), D2T1 (10% DMSO + 50 mM trehalose), D2T2 (10% DMSO + 100 mol trehalose) and no addition group D0T0 (0% DMSO + 0 mM trehalose). Diluted semen was frozen in granules in liquid nitrogen vapour. After thawing in 40°C water bath, determinate sperm livability, acrosome integrity, plasma membrane integrity, catalase (CAT) activity, superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) activity, malondialdehyde (MDA) content and reactive oxygen species (ROS) level, and the results showed that 10% DMSO and 0.05 mol/L trehalose could significantly improve the sperm motility, acrosome integrity rate and plasma membrane integrity rate after thawing, and significantly increase the levels of antioxidant enzymes CAT, SOD and GSH-Px in seminal plasma (P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 09712119 0974-1844 0971-2119 |
| Relation: | https://doaj.org/toc/0971-2119; https://doaj.org/toc/0974-1844 |
| DOI: | 10.1080/09712119.2022.2161551 |
| URL الوصول: | https://doaj.org/article/fcddba84c31f4bc6b92921bd677982e3 |
| رقم الانضمام: | edsdoj.fcddba84c31f4bc6b92921bd677982e3 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 09712119 09741844 |
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| DOI: | 10.1080/09712119.2022.2161551 |