Academic Journal
أعرض في EDS

PCR-based gene synthesis with overlapping unisense-oligomers asymmetric extension supported by a simulator for oligonucleotide extension achieved 1 kbp dsDNA

التفاصيل البيبلوغرافية
العنوان: PCR-based gene synthesis with overlapping unisense-oligomers asymmetric extension supported by a simulator for oligonucleotide extension achieved 1 kbp dsDNA
المؤلفون: Yasunori Nishida, Kotetsu Kayama, Taichi Endoh, Kiwamu Hanazono, Gerry Amor Camer, Daiji Endoh
المصدر: BioTechniques, Vol 74, Iss 6, Pp 317-332 (2023)
بيانات النشر: Taylor & Francis Group, 2023.
سنة النشر: 2023
المجموعة: LCC:Biology (General)
مصطلحات موضوعية: AESOE, optimization-free oligomer joining, synthetic genes, unisense joining oligomers, Biology (General), QH301-705.5
الوصف: We formulated a method to synthesize 1 kbp DNA fragments using ‘oligomer unidirectional joining method’ via asymmetric extension supported by a simulator for oligonucleotide extension (AESOE). In this study, trials were conducted on 41 sets of different genomic pieces of ten flaviviral genomes, and 31 bacterial 16s rRNA fragments with sizes ranging from 500 bases to 1.0 kbp. Synthetic gene production was found to be successful in all those sets. The synthesis method has three steps: the first step is a seven-linked AESOE, the second step is the linking of the 400-base fragments from the first step, and the third step is the final amplification. Our present approach is highly reproducible and may no longer require optimization of oligomer design.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1940-9818
0736-6205
Relation: https://doaj.org/toc/0736-6205; https://doaj.org/toc/1940-9818
DOI: 10.2144/btn-2022-0127
URL الوصول: https://doaj.org/article/fbf481aee640489db6e1383d79b060a2
رقم الانضمام: edsdoj.fbf481aee640489db6e1383d79b060a2
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19409818
07366205
DOI:10.2144/btn-2022-0127