التفاصيل البيبلوغرافية
| العنوان: |
RNA-binding proteins to assess gene expression states of co-cultivated cells in response to tumor cells |
| المؤلفون: |
Penalva Luiz OF, Burdick Michael D, Lin Simon M, Sutterluety Hedwig, Keene Jack D |
| المصدر: |
Molecular Cancer, Vol 3, Iss 1, p 24 (2004) |
| بيانات النشر: |
BMC, 2004. |
| سنة النشر: |
2004 |
| المجموعة: |
LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| مصطلحات موضوعية: |
Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| الوصف: |
Abstract Background Tumors and complex tissues consist of mixtures of communicating cells that differ significantly in their gene expression status. In order to understand how different cell types influence one another's gene expression, it will be necessary to monitor the mRNA profiles of each cell type independently and to dissect the mechanisms that regulate their gene expression outcomes. Results In order to approach these questions, we have used RNA-binding proteins such as ELAV/Hu, poly (A) binding protein (PABP) and cap-binding protein (eIF-4E) as reporters of gene expression. Here we demonstrate that the epitope-tagged RNA binding protein, PABP, expressed separately in tumor cells and endothelial cells can be used to discriminate their respective mRNA targets from mixtures of these cells without significant mRNA reassortment or exchange. Moreover, using this approach we identify a set of endothelial genes that respond to the presence of co-cultured breast tumor cells. Conclusion RNA-binding proteins can be used as reporters to elucidate components of operational mRNA networks and operons involved in regulating cell-type specific gene expression in tissues and tumors. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1476-4598 |
| Relation: |
http://www.molecular-cancer.com/content/3/1/24; https://doaj.org/toc/1476-4598 |
| DOI: |
10.1186/1476-4598-3-24 |
| URL الوصول: |
https://doaj.org/article/cdfbed83112c456aa8a0b98e9760db4f |
| رقم الانضمام: |
edsdoj.fbed83112c456aa8a0b98e9760db4f |
| قاعدة البيانات: |
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