Academic Journal
A high-precision wound healing assay based on photosensitized culture substrates
| العنوان: | A high-precision wound healing assay based on photosensitized culture substrates |
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| المؤلفون: | Saphia Azzam, Lea Tomasova, Carina Danner, Michael Skiba, Maren Klein, Zeno Guttenberg, Stefanie Michaelis, Joachim Wegener |
| المصدر: | Scientific Reports, Vol 14, Iss 1, Pp 1-14 (2024) |
| بيانات النشر: | Nature Portfolio, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Cell migration, Wound healing, Photosensitizer, Singlet oxygen, Reactive oxygen species, Phototoxicity, Medicine, Science |
| الوصف: | Abstract Quantitative assessment of cell migration in vitro is often required in fundamental and applied research from different biomedical areas including wound repair, tumor metastasis or developmental biology. A collection of assays has been established throughout the years like the most widely used scratch assay or the so-called barrier assay. It is the principle of these assays to introduce a lesion into an otherwise confluent monolayer in order to study the migration of cells from the periphery into this artificial wound and determine the migration rate from the time necessary for wound closure. A novel assay makes use of photosensitizers doped into a polystyrene matrix. A thin layer of this composite material is coated on the bottom of regular cell culture ware showing perfect biocompatibility. When adherent cells are grown on this coating, resonant excitation of the photosensitizer induces a very local generation of 1O2, which kills the cells residing at the site of illumination. Cells outside the site of illumination are not harmed. When excitation of the photosensitizer is conducted by microscopic illumination, high-precision wounding in any size and geometry is available even in microfluidic channels. Besides proof-of-concept experiments, this study gives further insight into the mechanism of photosensitizer-mediated cell wounding. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2045-2322 |
| Relation: | https://doaj.org/toc/2045-2322 |
| DOI: | 10.1038/s41598-024-59564-9 |
| URL الوصول: | https://doaj.org/article/afba277282b44a22bedb43c59134a97c |
| رقم الانضمام: | edsdoj.fba277282b44a22bedb43c59134a97c |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-024-59564-9 |