التفاصيل البيبلوغرافية
| العنوان: |
Old Yellow Enzyme from Trypanosoma cruzi Exhibits In Vivo Prostaglandin F2α Synthase Activity and Has a Key Role in Parasite Infection and Drug Susceptibility |
| المؤلفون: |
Florencia Díaz-Viraqué, María Laura Chiribao, Andrea Trochine, Fabiola González-Herrera, Christian Castillo, Ana Liempi, Ulrike Kemmerling, Juan Diego Maya, Carlos Robello |
| المصدر: |
Frontiers in Immunology, Vol 9 (2018) |
| بيانات النشر: |
Frontiers Media S.A., 2018. |
| سنة النشر: |
2018 |
| المجموعة: |
LCC:Immunologic diseases. Allergy |
| مصطلحات موضوعية: |
Trypanosoma cruzi, prostaglandin F2α synthase, Old Yellow Enzyme, differentially expressed gene, benznidazol and nifurtimox activation, Immunologic diseases. Allergy, RC581-607 |
| الوصف: |
The discovery that trypanosomatids, unicellular organisms of the order Kinetoplastida, are capable of synthesizing prostaglandins raised questions about the role of these molecules during parasitic infections. Multiple studies indicate that prostaglandins could be related to the infection processes and pathogenesis in trypanosomatids. This work aimed to unveil the role of the prostaglandin F2α synthase TcOYE in the establishment of Trypanosoma cruzi infection, the causative agent of Chagas disease. This chronic disease affects several million people in Latin America causing high morbidity and mortality. Here, we propose a prokaryotic evolutionary origin for TcOYE, and then we used in vitro and in vivo experiments to show that T. cruzi prostaglandin F2α synthase plays an important role in modulating the infection process. TcOYE overexpressing parasites were less able to complete the infective cycle in cell culture infections and increased cardiac tissue parasitic load in infected mice. Additionally, parasites overexpressing the enzyme increased PGF2α synthesis from arachidonic acid. Finally, an increase in benznidazole and nifurtimox susceptibility in TcOYE overexpressing parasites showed its participation in activating the currently anti-chagasic drugs, which added to its observed ability to confer resistance to hydrogen peroxide, highlights the relevance of this enzyme in multiple events including host–parasite interaction. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1664-3224 |
| Relation: |
http://journal.frontiersin.org/article/10.3389/fimmu.2018.00456/full; https://doaj.org/toc/1664-3224 |
| DOI: |
10.3389/fimmu.2018.00456 |
| URL الوصول: |
https://doaj.org/article/fa0bd5f7ee1e49a086af8d6ae1eb2a40 |
| رقم الانضمام: |
edsdoj.fa0bd5f7ee1e49a086af8d6ae1eb2a40 |
| قاعدة البيانات: |
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