Academic Journal
A continuum of zinc finger transcription factor retention on native chromatin underlies dynamic genome organization
| العنوان: | A continuum of zinc finger transcription factor retention on native chromatin underlies dynamic genome organization |
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| المؤلفون: | Siling Hu, Yangying Liu, Qifan Zhang, Juan Bai, Chenhuan Xu |
| المصدر: | Molecular Systems Biology, Vol 20, Iss 7, Pp 799-824 (2024) |
| بيانات النشر: | Springer Nature, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Biology (General) LCC:Medicine (General) |
| مصطلحات موضوعية: | Native Chromatin, Chromatin Structure, CTCF, Transcription Factor, ChIP, Biology (General), QH301-705.5, Medicine (General), R5-920 |
| الوصف: | Abstract Transcription factor (TF) residence on chromatin translates into quantitative transcriptional or structural outcomes on genome. Commonly used formaldehyde crosslinking fixes TF-DNA interactions cumulatively and compromises the measured occupancy level. Here we mapped the occupancy level of global or individual zinc finger TFs like CTCF and MAZ, in the form of highly resolved footprints, on native chromatin. By incorporating reinforcing perturbation conditions, we established S-score, a quantitative metric to proxy the continuum of CTCF or MAZ retention across different motifs on native chromatin. The native chromatin-retained CTCF sites harbor sequence features within CTCF motifs better explained by S-score than the metrics obtained from other crosslinking or native assays. CTCF retention on native chromatin correlates with local SUMOylation level, and anti-correlates with transcriptional activity. The S-score successfully delineates the otherwise-masked differential stability of chromatin structures mediated by CTCF, or by MAZ independent of CTCF. Overall, our study established a paradigm continuum of TF retention across binding sites on native chromatin, explaining the dynamic genome organization. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1744-4292 |
| Relation: | https://doaj.org/toc/1744-4292 |
| DOI: | 10.1038/s44320-024-00038-5 |
| URL الوصول: | https://doaj.org/article/ef9159d029ef4f10a05f703ec9f7e814 |
| رقم الانضمام: | edsdoj.f9159d029ef4f10a05f703ec9f7e814 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17444292 |
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| DOI: | 10.1038/s44320-024-00038-5 |