Academic Journal
Serological and molecular techniques applied for identification of Plasmodium spp. in blood samples from nonhuman primates
| العنوان: | Serological and molecular techniques applied for identification of Plasmodium spp. in blood samples from nonhuman primates |
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| المؤلفون: | Mayra Araguaia Pereira Figueiredo, Silvia Maria Di Santi, Wilson Gómez Manrique, Marcos Rogério André, Rosangela Zacarias Machado |
| المصدر: | Revista Brasileira de Parasitologia Veterinária, Vol 27, Iss 3, Pp 363-376 (2018) |
| بيانات النشر: | Colégio Brasileiro de Parasitologia Veterinaria, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Animal culture |
| مصطلحات موضوعية: | Malária simiana, macacos do Novo Mundo, Plasmodium brasilianum, Plasmodium malariae, 18S rRNA, zoonoses, Animal culture, SF1-1100 |
| الوصف: | Abstract The aim of this study was to identify Plasmodium spp. in blood samples from nonhuman primates (NHPs) in the state of Maranhão, using classical and alternative techniques for examination of human malaria. A total of 161 blood samples from NHPs were analyzed: 141 from captive animals at a Wildlife Screening Center (CETAS) and 20 from free-living animals in a private reserve. The techniques used were microscopy, rapid diagnostic test (RDT), Indirect fluorescent antibody test (IFAT) and molecular techniques (semi-nested PCR, quantitative real-time PCR and LAMP). Two serological methods (dot-ELISA and indirect ELISA) were also standardized with rhoptry protein-soluble antigen of P. falciparum and P. berghei. Trophozoite forms of Plasmodium sp. were identified on slides from five different animals. No samples were positive through RDT and LAMP. Four samples were seropositive for P. malariae through IFAT. The samples showed low reactivity to ELISA. Plasmodium sp. was detected in 34.16% (55/161) of the samples using qPCR based on the 18S rRNA gene. After sequencing, two samples showed 100% identityl to P. malariae, one showed 97% identity to Plasmodium sp. ZOOBH and one showed 99% identity to P. falciparum . PCR was shown to be the most sensitive technique for diagnosing Plasmodium in NHP samples. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Spanish; Castilian Portuguese |
| تدمد: | 1984-2961 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000300363&lng=en&tlng=en; https://doaj.org/toc/1984-2961 |
| DOI: | 10.1590/s1984-296120180043 |
| URL الوصول: | https://doaj.org/article/f725417a6ab04f569b02d22347b63ad4 |
| رقم الانضمام: | edsdoj.f725417a6ab04f569b02d22347b63ad4 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 19842961 |
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| DOI: | 10.1590/s1984-296120180043 |