التفاصيل البيبلوغرافية
| العنوان: |
Gold Nanoparticles-Based Colorimetric Immunoassay of Carcinoembryonic Antigen with Metal–Organic Framework to Load Quinones for Catalytic Oxidation of Cysteine |
| المؤلفون: |
Zhao-Jiang Yu, Ting-Ting Yang, Gang Liu, De-Hua Deng, Lin Liu |
| المصدر: |
Sensors, Vol 24, Iss 20, p 6701 (2024) |
| بيانات النشر: |
MDPI AG, 2024. |
| سنة النشر: |
2024 |
| المجموعة: |
LCC:Chemical technology |
| مصطلحات موضوعية: |
colorimetric immunoassays, gold nanoparticles, redox cycling, metal–organic frameworks, quinone, cysteine, Chemical technology, TP1-1185 |
| الوصف: |
This work reported gold nanoparticles (AuNPs)-based colorimetric immunoassay with the Cu-based metal–organic framework (MOF) to load pyrroloquinoline quinone (PQQ) for the catalytic oxidation of cysteine. In this method, both Cu2+ and PQQ in the MOF could promote the oxidation of inducer cysteine by redox cycling, thus limiting the cysteine-induced aggregation of AuNPs and achieving dual signal amplification. Specifically, the recombinant carcinoembryonic antigen (CEA) targets were anchored on the MOF through the metal coordination interactions between the hexahistidine (His6) tag in CEA and the unsaturated Cu2+ sites in MOF. The CEA/PQQ-loaded MOF could be captured by the antibody-coated ELISA plate to catalyze the oxidation of cysteine. However, once the target CEA in the samples bound to the antibody immobilized on the plate surface, the attachment of CEA/PQQ-loaded MOF would be limited. Cysteine remaining in the solution would trigger the aggregation of AuNPs and cause a color change from red to blue. The target concentration was positively related to the aggregation and color change of AuNPs. The signal-on competitive plasmonic immunoassay exhibited a low detection limit with a linear range of 0.01–1 ng/mL. Note that most of the proteins in commercial ELISA kits are recombinant with a His6 tag in the N- or C-terminal, so the work could provide a sensitive plasmonic platform for the detection of biomarkers. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1424-8220 |
| Relation: |
https://www.mdpi.com/1424-8220/24/20/6701; https://doaj.org/toc/1424-8220 |
| DOI: |
10.3390/s24206701 |
| URL الوصول: |
https://doaj.org/article/f7161754c0164827830aa84ac5ba4290 |
| رقم الانضمام: |
edsdoj.f7161754c0164827830aa84ac5ba4290 |
| قاعدة البيانات: |
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