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A Multiweek Project Examining the Chemotactic Behavior of Tetrahymena in an Undergraduate Biology Laboratory

التفاصيل البيبلوغرافية
العنوان: A Multiweek Project Examining the Chemotactic Behavior of Tetrahymena in an Undergraduate Biology Laboratory
المؤلفون: Rachel Hongo, Robert T. Grammer, Christopher E. Barton
المصدر: Journal of Microbiology & Biology Education, Vol 21, Iss 1 (2020)
بيانات النشر: American Society for Microbiology, 2020.
سنة النشر: 2020
المجموعة: LCC:Special aspects of education
LCC:Biology (General)
مصطلحات موضوعية: Special aspects of education, LC8-6691, Biology (General), QH301-705.5
الوصف: A multi-week laboratory project has been developed to incorporate elements of student investigation, chemotactic behavior of protists, and genetic effects on chemotactic activity of Tetrahymena. The chemotaxis assay is based on spectrophotometric detection of protist-induced light scattering as cells migrate into a density gradient containing a known attractant. The three-week project consists of an introductory chemotaxis assay, investigation of dose-response effects, and culminates with the exploration of a Tetrahymena genetic mutant with known defects in motility. Additionally, this project incorporates a microscopic investigation of cellular structure and swimming behavior of mutant and wild-type cells. Students have responded well to the nature of the project, displaying clear understanding of the mechanism of the assay as well as the response of the protists to environmental manipulation and the molecular defects in the mutant cells.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1935-7885
1935-7877
Relation: https://doaj.org/toc/1935-7877; https://doaj.org/toc/1935-7885
DOI: 10.1128/jmbe.v21i1.1805
URL الوصول: https://doaj.org/article/f6e1fe7a3ca0452c9867c43b46e17a7a
رقم الانضمام: edsdoj.f6e1fe7a3ca0452c9867c43b46e17a7a
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19357885
19357877
DOI:10.1128/jmbe.v21i1.1805