Academic Journal
A replication-competent smallpox vaccine LC16m8Δ-based COVID-19 vaccine
| العنوان: | A replication-competent smallpox vaccine LC16m8Δ-based COVID-19 vaccine |
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| المؤلفون: | Akihiko Sakamoto, Hiroaki Osawa, Hinata Hashimoto, Tetsushi Mizuno, Ammar A. Hasyim, Yu-ichi Abe, Yuto Okahashi, Ryohei Ogawa, Mitsuhiro Iyori, Hisatoshi Shida, Shigeto Yoshida |
| المصدر: | Emerging Microbes and Infections, Vol 11, Iss 1, Pp 2359-2370 (2022) |
| بيانات النشر: | Taylor & Francis Group, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Infectious and parasitic diseases LCC:Microbiology |
| مصطلحات موضوعية: | BALB/c mouse, cellular immunity, COVID-19, Delta/B.1.617.2 variant of concern, humoral immunity, LC16m8, Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502 |
| الوصف: | Viral vectors are a potent vaccine platform for inducing humoral and T-cell immune responses. Among the various viral vectors, replication-competent ones are less commonly used for coronavirus disease 2019 (COVID-19) vaccine development compared with replication-deficient ones. Here, we show the availability of a smallpox vaccine LC16m8Δ (m8Δ) as a replication-competent viral vector for a COVID-19 vaccine. M8Δ is a genetically stable variant of the licensed and highly effective Japanese smallpox vaccine LC16m8. Here, we generated two m8Δ recombinants: one harbouring a gene cassette encoding the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) glycoprotein, named m8Δ-SARS2(P7.5-S)-HA; and one encoding the S protein with a highly polybasic motif at the S1/S2 cleavage site, named m8Δ-SARS2(P7.5-SHN)-HA. M8Δ-SARS2(P7.5-S)-HA induced S-specific antibodies in mice that persisted for at least six weeks after a homologous boost immunization. All eight analysed serum samples displayed neutralizing activity against an S-pseudotyped virus at a level similar to that of serum samples from patients with COVID-19, and more than half (5/8) also had neutralizing activity against the Delta/B.1.617.2 variant of concern. Importantly, most serum samples also neutralized the infectious SARS-CoV-2 Wuhan and Delta/B.1.617.2 strains. In contrast, immunization with m8Δ-SARS2(P7.5-SHN)-HA elicited significantly lower antibody titres, and the induced antibodies had less neutralizing activity. Regarding T-cell immunity, both m8Δ recombinants elicited S-specific multifunctional CD8+ and CD4+ T-cell responses even after just a primary immunization. Thus, m8Δ provides an alternative method for developing a novel COVID-19 vaccine. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 22221751 2222-1751 |
| Relation: | https://doaj.org/toc/2222-1751 |
| DOI: | 10.1080/22221751.2022.2122580 |
| URL الوصول: | https://doaj.org/article/f6ad526e39bc48c29e6dd9daa91b7d72 |
| رقم الانضمام: | edsdoj.f6ad526e39bc48c29e6dd9daa91b7d72 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22221751 |
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| DOI: | 10.1080/22221751.2022.2122580 |