التفاصيل البيبلوغرافية
| العنوان: |
The CXCL5/CXCR2 axis contributes to the epithelial-mesenchymal transition of nasopharyngeal carcinoma cells by activating ERK/GSK-3β/snail signalling |
| المؤلفون: |
Wen-Ze Qiu, Hai-Bo Zhang, Wei-Xiong Xia, Liang-Ru Ke, Jing Yang, Ya-Hui Yu, Hu Liang, Xin-Jun Huang, Guo-Ying Liu, Wang-Zhong Li, Yan-Qun Xiang, Tie-Bang Kang, Xiang Guo, Xing Lv |
| المصدر: |
Journal of Experimental & Clinical Cancer Research, Vol 37, Iss 1, Pp 1-17 (2018) |
| بيانات النشر: |
BMC, 2018. |
| سنة النشر: |
2018 |
| المجموعة: |
LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| مصطلحات موضوعية: |
CXCL5, CXCR2, Epithelial-to-mesenchymal transition, Distant metastasis, Nasopharyngeal carcinoma, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| الوصف: |
Abstract Background Distant metastasis is the major cause of treatment failure in patients with nasopharyngeal carcinoma (NPC). Although several biomarkers correlate with metastasis and prognosis, the molecular mechanisms of NPC development and progression remain unclear. Methods Quantitative RT-PCR (qRT-PCR), western blotting, cell growth, foci formation, migration and invasion assays, and xenograft mouse models were utilized to examine the expression levels and functions of the CXCL5/CXCR2 axis in NPC. A luciferase reporter assay, western blotting, immunofluorescence, and migration and invasion assays were used to identify and verify the ERK/GSK-3β/Snail signalling pathway. Results CXCL5 was significantly increased in the sera of NPC patients, and high expression levels of CXCL5/CXCR2 in NPC primary tissues indicated poor survival. CXCL5 and CXCR2 were upregulated in NPC cell lines. Ectopic expression of the CXCL5/CXCR2 axis promoted NPC cell migration and invasion in vitro and the formation of lung metastases in vivo. Mechanistically, the dual overexpression of CXCL5 and CXCR2 promoted cell spreading by inducing the epithelial-mesenchymal transition (EMT) through the activation of the ERK/GSK-3β/Snail signalling pathway. Conclusion The CXCL5/CXCR2 axis contributes to the EMT of NPC cells by activating ERK/GSK-3β/Snail signalling, and this axis may be a potential diagnostic marker and therapeutic target for patients with NPC. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1756-9966 |
| Relation: |
http://link.springer.com/article/10.1186/s13046-018-0722-6; https://doaj.org/toc/1756-9966 |
| DOI: |
10.1186/s13046-018-0722-6 |
| URL الوصول: |
https://doaj.org/article/df67b8d2c1934e4d97a7674a4441b0d8 |
| رقم الانضمام: |
edsdoj.f67b8d2c1934e4d97a7674a4441b0d8 |
| قاعدة البيانات: |
Directory of Open Access Journals |
Full Text Finder