التفاصيل البيبلوغرافية
| العنوان: |
Transcriptomic meta-analysis identifies gene expression characteristics in various samples of HIV-infected patients with nonprogressive disease |
| المؤلفون: |
Le-Le Zhang, Zi-Ning Zhang, Xian Wu, Yong-Jun Jiang, Ya-Jing Fu, Hong Shang |
| المصدر: |
Journal of Translational Medicine, Vol 15, Iss 1, Pp 1-10 (2017) |
| بيانات النشر: |
BMC, 2017. |
| سنة النشر: |
2017 |
| المجموعة: |
LCC:Medicine |
| مصطلحات موضوعية: |
HIV-1, LTNP, EC, Integrative transcriptome analyses, Microarray, Meta-analysis, Medicine |
| الوصف: |
Abstract Background A small proportion of HIV-infected patients remain clinically and/or immunologically stable for years, including elite controllers (ECs) who have undetectable viremia (10 years). However, the mechanism of nonprogression needs to be further resolved. In this study, a transcriptome meta-analysis was performed on nonprogressor and progressor microarray data to identify differential transcriptome pathways and potential biomarkers. Methods Using the INMEX (integrative meta-analysis of expression data) program, we performed the meta-analysis to identify consistently differentially expressed genes (DEGs) in nonprogressors and further performed functional interpretation (gene ontology analysis and pathway analysis) of the DEGs identified in the meta-analysis. Five microarray datasets (81 cases and 98 controls in total), including whole blood, CD4+ and CD8+ T cells, were collected for meta-analysis. Results We determined that nonprogressors have reduced expression of important interferon-stimulated genes (ISGs), CD38, lymphocyte activation gene 3 (LAG-3) in whole blood, CD4+ and CD8+ T cells. Gene ontology (GO) analysis showed a significant enrichment in DEGs that function in the type I interferon signaling pathway. Upregulated pathways, including the PI3K-Akt signaling pathway in whole blood, cytokine–cytokine receptor interaction in CD4+ T cells and the MAPK signaling pathway in CD8+ T cells, were identified in nonprogressors compared with progressors. In each metabolic functional category, the number of downregulated DEGs was more than the upregulated DEGs, and almost all genes were downregulated DEGs in the oxidative phosphorylation (OXPHOS) and tricarboxylic acid (TCA) cycle in the three types of samples. Conclusions Our transcriptomic meta-analysis provides a comprehensive evaluation of the gene expression profiles in major blood types of nonprogressors, providing new insights in the understanding of HIV pathogenesis and developing strategies to delay HIV disease progression. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1479-5876 |
| Relation: |
http://link.springer.com/article/10.1186/s12967-017-1294-5; https://doaj.org/toc/1479-5876 |
| DOI: |
10.1186/s12967-017-1294-5 |
| URL الوصول: |
https://doaj.org/article/f552ba4833934792af11a0c4906b7de8 |
| رقم الانضمام: |
edsdoj.f552ba4833934792af11a0c4906b7de8 |
| قاعدة البيانات: |
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