Academic Journal
Stress induces divergent gene expression among lateral habenula efferent pathways
| العنوان: | Stress induces divergent gene expression among lateral habenula efferent pathways |
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| المؤلفون: | Marjorie R. Levinstein, Kevin R. Coffey, Russell G. Marx, Atom J. Lesiak, John F. Neumaier |
| المصدر: | Neurobiology of Stress, Vol 13, Iss , Pp 100268- (2020) |
| بيانات النشر: | Elsevier, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Neurosciences. Biological psychiatry. Neuropsychiatry LCC:Neurology. Diseases of the nervous system LCC:Neurophysiology and neuropsychology |
| مصطلحات موضوعية: | Lateral habenula, Gene expression, RiboTag, rnaSEQ, PI3K, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571, Neurology. Diseases of the nervous system, RC346-429, Neurophysiology and neuropsychology, QP351-495 |
| الوصف: | The lateral habenula (LHb) integrates critical information regarding aversive stimuli that shapes decision making and behavioral responses. The three major LHb outputs innervate dorsal raphe nucleus (DRN), ventral tegmental area (VTA), and the rostromedial tegmental nucleus (RMTg). LHb neurons that project to these targets are segregated and nonoverlapping, and this led us to consider whether they have distinct molecular phenotypes and adaptations to stress exposure. In order to capture a time-locked profile of gene expression after repeated forced swim stress, we used intersectional expression of RiboTag in rat LHb neurons and next-gen RNA sequencing to interrogate the RNAs actively undergoing translation from each of these pathways. The “translatome” in the neurons comprising these pathways was similar at baseline, but diverged after stress, especially in the neurons projecting to the RMTg. Using weighted gene co-expression network analysis, we found one module, which had an overrepresentation of genes associated with phosphoinositide 3 kinase (PI3K) signaling, comprising genes downregulated after stress in the RMTg-projecting LHb neurons. Reduced PI3K signaling in RMTg-projecting LHb neurons may be a compensatory adaptation that alters the functional balance of LHb outputs to GABAergic vs. monoaminergic neurons following repeated stress exposure. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2352-2895 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2352289520300588; https://doaj.org/toc/2352-2895 |
| DOI: | 10.1016/j.ynstr.2020.100268 |
| URL الوصول: | https://doaj.org/article/f3a8de9ef1304d5083824b089e1e3a85 |
| رقم الانضمام: | edsdoj.f3a8de9ef1304d5083824b089e1e3a85 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23522895 |
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| DOI: | 10.1016/j.ynstr.2020.100268 |