Academic Journal
Ultrasonication-mediated synthesis of diblock polymer-based nanoparticles for advanced drug delivery systems: Insights and optimization
| العنوان: | Ultrasonication-mediated synthesis of diblock polymer-based nanoparticles for advanced drug delivery systems: Insights and optimization |
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| المؤلفون: | Nagaraja Sreeharsha, Srikruthi Kunigal Sridhar, Asha Bhuvanahalli Rangappa, Prakash Goudanavar, Purushotham Karadigere Nagaraju, Nimbagal Raghavendra Naveen, Predeepkumar Narayanappa Shiroorkar, Afzal Haq Asif, Girish Meravanige, Krishna Swaroop Duddi Sreehari |
| المصدر: | Ultrasonics Sonochemistry, Vol 111, Iss , Pp 107137- (2024) |
| بيانات النشر: | Elsevier, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Chemistry LCC:Acoustics. Sound |
| مصطلحات موضوعية: | Diblock Polymer, Ultrasonication, Solid Lipid Nanoparticles, Dolutegravir, Optimization, Chemistry, QD1-999, Acoustics. Sound, QC221-246 |
| الوصف: | This study presents the synthesis and optimization of Methylene polyethyl glycol −Polystyrene (mPEG-PS) Diblock (DIP) copolymer-based solid lipid nanoparticles (SLNs) using ultrasonication for advanced drug delivery systems targeting the human immunodeficiency virus (HIV-1). The mPEG-PS block copolymer was synthesized by ring opening polymerization mechanism under nitrogen atmosphere for 24hrs and characterized using Fourier Transform Infrared Spectroscopy (FTIR) spectroscopy and NMR, confirming the formation of DIP polymers. Optimization of SLNs formulation was achieved through a systematic approach, utilizing response surface methodology, optimal conditions for SLNs synthesis were determined, resulting in nanoparticles with a particle size of 198 nm and an entrapment efficiency of 67.42 %. Cell viability assays, quantitative PCR for viral DNA analysis, caspase-3 enzyme assays, and quantitative uptake studies using High Performance Liquid Chromatography (HPLC) provided quantitative insights into the efficacy and biocompatibility of the synthesized nanoparticles. The experimental data demonstrate that nanoparticle treatments significantly influence cellular responses, providing valuable insights into their therapeutic potential and underlying mechanisms. By employing precise experimental methods alongside rigorous analytical techniques, this study enhances our understanding of nanoparticle-based drug delivery systems, particularly in the context of HIV treatment. These findings pave the way for optimizing therapeutic strategies to improve patient outcomes. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1350-4177 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S1350417724003869; https://doaj.org/toc/1350-4177 |
| DOI: | 10.1016/j.ultsonch.2024.107137 |
| URL الوصول: | https://doaj.org/article/f2852736ea864fc99c3caf3c66baa1ed |
| رقم الانضمام: | edsdoj.f2852736ea864fc99c3caf3c66baa1ed |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 13504177 |
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| DOI: | 10.1016/j.ultsonch.2024.107137 |